Graduate School of Pharmaceutical Science, Osaka University
Graduate School of Pharmaceutical Science, Osaka University
Graduate School of Pharmaceutical Science, Osaka University
Graduate School of Pharmaceutical Science, Osaka University
出版者
宇宙航空研究開発機構宇宙科学研究所 (JAXA)(ISAS)
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)
雑誌名
宇宙利用シンポジウム: 第27回: 平成22年度
雑誌名(英)
Space Utilization Research, Vol. 27 2011: Proceedings of The Twenty-seventh Space Utilization Symposium
The Twenty-seventh Space Utilization Symposium (January 24-25, 2011. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan
抄録（英）
Microbes exist everywhere, even in a space habitat. The microbial ecosystem may differ from those on Earth in such a closed environment under microgravity. It is necessary to investigate the relationship between humans and microbes, and how microbes influence the systems and materials in this environment. The objective of our experiment is to monitor the abundance of bacteria and predict their dynamics in "KIBO", the Japanese experiment module of the International Space Station (ISS), from our own viewpoints. This time, we measured the bacterial abundance on a multi protocol converter (MPC) used in the ISS. Bacterial cells on the surfaces and parts outside and inside of a MPC were collected with swab or "microbecollecting adhesive sheet (Yamaguchi, et al. J. Microbial. Methods, 53: 405, 2003)", and bacterial number was measured by quantitative realtime PCR targeting bacterial 16S rRNA gene sequences following DNAextraction from bacterial cells collected with swab or sampling sheet. Sampling method with the adhesive sheet is simple, rapid, and reproducible. This sheet does not require water to recover the bacteria from the targeted surface, while the swab method needs water to moisturize the head. This sampling sheet is suitable to collect bacterial cells on solid surfaces and we believe this sheet would be useful device in the field of astrobiology.
内容記述
形態: カラー図版あり
内容記述（英）
Physical characteristics: Original contains color illustrations
国際宇宙ステーションから回収した機器における細菌の現存量
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