Graduate School of Biosphere Science, Hiroshima University
Graduate School of Biosphere Science, Hiroshima University
Graduate School of Biosphere Science, Hiroshima University
Graduate School of Biosphere Science, Hiroshima University
Graduate School of Biosphere Science, Hiroshima University
Graduate School of Biosphere Science, Hiroshima University
Faculty of Appled Biological Science, Hiroshima University,
Faculty of Appled Biological Science, Hiroshima University,
Graduate School of Biosphere Science, Hiroshima University
Bioscience Group, National Institute of Polar Research
Japan Atomic Energy Agency (JAEA)
Graduate School of Engineering, Yokohama National University
High Energy Accelerator Research Organization (KEK)
Graduate School of Engineering, Yokohama National University
High Energy Accelerator Research Organization (KEK)
Graduate School of Natural Science and Technology, Okayama University
Japan Aerospace Exploration Agency (JAXA)
High Energy Accelerator Research Organization (KEK)
Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
High Energy Accelerator Research Organization (KEK)
High Energy Accelerator Research Organization (KEK)
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)
High Energy Accelerator Research Organization (KEK)
National Institute of Radiological Sciences
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (ISAS)(JAXA)
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
雑誌名
宇宙利用シンポジウム
雑誌名(英)
Space Utilization Research: Proceedings of Space Utilization Symposium
The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan)
抄録（英）
One of major problems in astrobiology is intense radiation, theoretical and experimental studies on biological protection and tolerance to radiation are needed. Wet and freeze-dried microbial cells were irradiated with high-dose X-ray for 0 to 10,000 Gy and high-energy Fe ions for 0 to 4,850 Gy to examine their survivability based on the most probably number (MPN), the biological "energy currency", i.e., adenosine 5'-triphosphate (ATP) and genomic DNA fragmentation. The well known radiation-resistant bacteria such as genus Deinococcus radiodurans, highly stress-resistant spore former Bacillus subtilis spore and radiation-sensitive Escherichia coli were used for this experiment. Irradiation of synchrotron X-ray (0.2 nm) at a high dose rate of 4.4 Gy/s (500 R/s) was done at KEK Photon Factory. Freeze dried D. raduidurans and E. coli cells showed higher survivability than their wet cells. But B. subtilis spores have not changed their survivability wet or freeze-dried. Freeze-dried celluar ATP showed no decrease against to the dose. In addition, genomic DNA fragmentation was not easy to generated in freeze-dried cells. Irradiation of 500 MeV Fe ion at a dose rate of 0.172 Gy/s (2.5×10(exp8) particles/s) was done at NIRS HIMAC. Generally similar results to X-ray irradiation were observed, but ATP showed exponential decrease in wet cells against to the dose.
内容記述
形態: カラー図版あり
共催: 日本学術会議
著者人数: 25人
内容記述（英）
Physical characteristics: Original contains color illustrations
joint hosting: The Science Council of Japan
大線量エックス線および高エネルギーFeイオン照射に対する微生物
64730053.pdf
(505.29KB)
