Graduate School of Science and Engineering, University of Toyama
Department of Biology and Geosciences, Graduate School of Science, Osaka City University
Department of Biology and Geosciences, Graduate School of Science, Osaka City University
Department of Biology and Geosciences, Graduate School of Science, Osaka City University
School of Engineering, Hokkaido Tokai University
University of Tokyo
Graduate School of Life Sciences, Tohoku University
Graduate School of Biological Sciences, Nara Institute of Science and Technology,
Graduate School of Life Sciences, Tohoku University
Depertment of Biological Science, Graduate School of Science, Osaka Prefecture University
Faculty of Liberal Arts and Sciences, Osaka Prefecture University
Graduate School Division of Science and Engineering, University of Toyama
Graduate School Division of Science and Engineering, University of Toyama
Graduate School Division of Science and Engineering, University of Toyama
Graduate School Division of Science and Engineering, University of Toyama
Graduate School Division of Science and Engineering, University of Toyama
Chiyoda Advanced Solutions Corporation
Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum
Japan Aerospace Exploration Agency (JAXA)
ISS Science Project Office, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)
Graduate School of Life & Environmental Science, Osaka Prefecture University
Institute for Environmental Sciences
Graduate School of Horticulture, Chiba University
Faculty of Agriculture, Kyushu University
Japan Manned Space Systems (JAMSS)
Advanced Science Research Center, Kanazawa University
Division of Functional Genomics, Advanced Science Research Center, Kanazawa University
Advanced Engineering Services
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者(英)
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
雑誌名
宇宙利用シンポジウム
雑誌名(英)
Space Utilization Research: Proceedings of Space Utilization Symposium
The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan)
抄録(英)
Plant life cycle consists of successive phases of vegetative and reproductive growth. The effects of altered gravity conditions on reproductive growth are not understood well compared to vegetative growth. We have performed proteome analysis to examine the effect of hypergravity on reproductive growth of Arabidopsis (Arabidopsis thaliana (L.) Heynh. ecotype Columbia-0). Plants grown for 20-26 days under were exposed to hypergravity at 300 x g or placed at 1 x g for 24 h in the dark. Proteins extracted from the flower buds with a buffer containing Triton X-100 were subjected to two-dimensional electrophoresis. The polypeptides, that were up-regulated or down-regulated by hypergravity, were analyzed by matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry, to compare the present proteome results with our previous transcriptome results. Proteome analysis showed that hypergravity changed the expression of proteins involved in protein folding and translocation, ethylene synthesis and stress response.