Tateyama Marine Laboratory, Marine and Coastal Research Center, Ochanomizu University
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA) (ISAS)
Research Center for Nuclear Science and Technology, The University of Tokyo
Tateyama Marine Laboratory, Marine and Coastal Research Center, Ochanomizu University
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
雑誌名
宇宙利用シンポジウム
雑誌名(英)
Space Utilization Research: Proceedings of Space Utilization Symposium
The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan)
抄録（英）
Sea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicules grow up to larval skeletons. The skeletogenic cells are called primary mesenchyme cells derived from micromeres at 16-cell stage. We already reported a promotive effect of hypergravity on skeletogenesis in the culture of the skeletogenic cells with low concentration of horse serum. In this study, we examined the culture condition, the component necessary for spicule formation. The low activity of gel filtration fraction was recovered by the addition of affinity heparin column flow through fraction. VEGF, one candidate of the factor for spicule formation, was not effective for micromere culture, even with low concentration of horse serum. These results show the possibility that two or more factors are necessary for spicule formation in the culture of micromeres.
内容記述
形態: カラー図版あり
共催: 日本学術会議
内容記述（英）
Physical characteristics: Original contains color illustrations
joint hosting: The Science Council of Japan
重力実験のためのウニの幼生骨格の培養系の検討
64730070.pdf
(930.52KB)
