Nara Medical University : Japan Aerospace Exploration Agency (JAXA)
Nara Medical University : Japan Aerospace Exploration Agency (JAXA)
Japan Aerospace Exploration Agency (JAXA) : Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum
Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum
Kagoshima University : Advanced Engineering Services (AES)
Kagoshima University : Advanced Engineering Services (AES)
ISS Science Project Office, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS) : Kagoshima University
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
雑誌名
宇宙利用シンポジウム
雑誌名(英)
Space Utilization Research: Proceedings of Space Utilization Symposium
The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan)
抄録（英）
A p53 tumor suppressor protein is generally thought to contribute to the genetic stability of cells against DNA damage through the activity of p53-centered signal transduction pathways. To clarify the effect of the space radiations on the gene expression of p53-dependent regulated genes, space experiments were performed with two human cultured lymphoblastoid cell lines: one cells line (TSCE5) bears a wild-type p53 gene status, and another cells line (WTK1) bears a mutated p53 gene status. Frozen human lymphoblastoid cells were stored on the International Space Station (ISS) for 133 days. The gene expression was analyzed by using DNA chip (a 44k whole human genome microarray, Agilent Technologies Inc.) after culturing the space samples for 6 h on the ground after their return from space. p53-Dependent up-regulated gene expression was found for 50 genes and p53-dependent down-regulated gene expression was found for 94 genes. The biological meanings of them were discussed from an aspect of the gene function about up- and down-regulated genes by the exposure of space radiations at low doses.
「きぼう」でのRad Gene 宇宙実験報告1 : ヒト培養系における宇宙飛行後のp53 依存性遺伝子発現
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