ageotropum Pea Mutant, Alaska Pea, Automorphosis, Auxin Polar Transport, Efflux Facilitator Protein, Growth and Development, Influx Facilitator Protein, PsAUX1, PsPINs
その他のタイトル（英）
PsPINs and PsAUX1 genes encoding putative facilitator proteins of an efflux and an influx of auxin, respectively, from etiolated epicotyls of an agravitropic pea (Pisum sativum L.) mutant, ageotropum
Graduate School of Science, Osaka Prefecture University
Graduate School of Science, Osaka Prefecture University
Graduate School of Science, Osaka Prefecture University
Faculty of Liberal Arts and Sciences, Osaka Prefecture University
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
雑誌名
宇宙利用シンポジウム
雑誌名(英)
Space Utilization Research: Proceedings of Space Utilization Symposium
The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan)
抄録（英）
We have isolated novel cDNAs containing the complete open reading frames of a putative auxin influx facilitator, PsAUX1, and putative auxin efflux facilitators, PsPIN1 and PsPIN2, from etiolated epicotyls of an agravitropic pea (Pisum sativum L.) mutant, ageotropum. High levels of homology were found on nucleotide and deduced amino acid sequences among PsPIN1 (Accession No. AY222857) and PsPIN2 (Accession No. AB112364) from an agravitropic pea mutant of ageotropum and a normal gravitropic pea of Alaska, AtPINs from Arabidopsis thaliana and others. Phylogenetic analyses based on deduced amino acid sequences revealed that PsPIN2 from ageotropum pea and Alaska pea belonged to a subclade including AtPIN3, AtPIN4 and AtPIN7, while PsPIN1 from ageotropum pea and Alaska pea belonged to the same clade as AtPIN1. The results were similar for PsAUX1 and AtAUX1, where PsAUX1 from ageotropum pea and Alaska pea belonged to the same subclade as AtAUX1 and CsAUX1. There was no deletion and insertion of nucleotides in these genes from ageotropum pea compared to those from Alaska pea. Expression of PsPIN1, PsP1N2 and PsAUX1 genes in the first internode of etiolated ageotropum and Alaska pea epicotyls were almost same during early growth stage of the seedlings. In contrast, ageotropum pea seedlings showed automorphosis-like growth observed in true and simulated microgravity conditions in space and on a three-dimensional clinostat, respectively. Auxin polar transport of the first internode of etiolated ageotroum pea epicotyls was substantially suppressed to lower than 50 % of that in etiolated Alaska pea epicotyls. These results suggest that reduced auxin polar transport in etiolated ageotropum pea epicotyls does not depend on structure and function, and gene expression of PsPINs and PsAUX1 but on different mode of actions of other molecules regulating auxin polar transport and/or dynamics of the products of PsPINs and PsAUX1 genes between ageotropum pea and Alaska pea.
エンドウ重力応答突然変異体"agetropum"におけるオーキシン極性移動関連遺伝子
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