WEKO3
アイテム
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Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoblasts and osteoclasts with AlkaLine Phosphatase (ALP) and Tartrate-Resistant Acid Phosphatase (TRAP) as the respective markers and analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we indicated that osteoblastic and osteoclastic activities in the scale responded to the simulated microgravity with a three-dimensional clinostat. As these activities in the scale did not change by the stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in the scale osteoblasts and osteoclasts is a specific phenomenon. In addition, we analyzed the bone metabolism under 2-, 4-, and 7-gravity (G) loading with a centrifuge and compared them with the control (1-G). The osteoblastic activity significantly increased under 2- and 4-G loading. This activity increased remarkably under 7-G loading. On the other hand, we found that the osteoclastic activity significantly decreased under 2-G loading. Under 4-G loading, there was no significant difference between G-loaded scales and control scales. The osteoclastic activity tended to increase under 7-G loading. These results were similar to our previous study of acceleration by vibration. With the goal of developing a drug for bone diseases, the effect of novel bromomelatonin derivatives on osteoblasts and osteoclasts was examined. All bromomelatonin derivatives had an inhibitory action on osteoclasts. In particular, 1-benzyl-2, 4, 6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action. In addition, estrogen receptor mRNA (messenger Ribonucleic Acid) expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(exp -7) M)-treated scales. 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擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析
https://jaxa.repo.nii.ac.jp/records/13435
https://jaxa.repo.nii.ac.jp/records/1343515f5144d-a32b-4120-9d32-21afc3307a0f
名前 / ファイル | ライセンス | アクション |
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63706059.pdf (379.5 kB)
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Item type | 会議発表論文 / Conference Paper(1) | |||||
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公開日 | 2015-03-26 | |||||
タイトル | ||||||
タイトル | 擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 骨芽細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 破骨細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 魚鱗 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 魚 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 代謝 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 細胞培養 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | クリノスタット | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 微小重力 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 遠心機 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 高重力環境 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ブロモメラトニン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 金魚 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 重力効果 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 活性 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | osteoblast | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | osteoclast | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | fish scale | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | fish | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | metabolism | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cell culturing | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | clinostat | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | microgravity | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | centrifuge | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | high gravity environment | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | bromomelatonin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Carassius auratus | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | gravitational effect | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | activity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
資源タイプ | conference paper | |||||
その他のタイトル(英) | ||||||
その他のタイトル | Regulation of bone metabolism under simulated micro-gravity and hyper-gravity loading: Analysis by the cultured scales of goldfish | |||||
著者 |
鈴木, 信雄
× 鈴木, 信雄× 大森, 克徳× 井尻, 憲一× 北村, 敬一郎× 清水, 宣明× 田畑, 純× 池亀, 美華× 中村, 正久× 近藤, 隆× 松田, 恒平× Suzuki, Nobuo× Omori, Katsunori× Ijiri, Kenichi× Kitamura, Keiichiro× Shimizu, Nobuaki× Tabata, Makoto J.× Ikegame, Mika× Nakamura, Masahisa× Kondo, Takashi× Matsuda, Kohei |
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著者所属 | ||||||
金沢大学 自然計測応用研究センター | ||||||
著者所属 | ||||||
宇宙航空研究開発機構 | ||||||
著者所属 | ||||||
東京大学 アイソトープ総合センター | ||||||
著者所属 | ||||||
金沢大学 大学院医学系研究科 | ||||||
著者所属 | ||||||
金沢大学 自然計測応用研究センター | ||||||
著者所属 | ||||||
東京医科歯科大学 大学院 | ||||||
著者所属 | ||||||
岡山大学 大学院医歯薬学総合研究科 | ||||||
著者所属 | ||||||
早稲田大学 教育・総合科学学術院 | ||||||
著者所属 | ||||||
富山大学 医学薬学研究部 | ||||||
著者所属 | ||||||
富山大学 大学院理工学研究科 | ||||||
著者所属(英) | ||||||
en | ||||||
Kanazawa University Institute of Nature and Environmental Technology | ||||||
著者所属(英) | ||||||
en | ||||||
Japan Aerospace Exploration Agency | ||||||
著者所属(英) | ||||||
en | ||||||
University of Tokyo Radioisotope Center | ||||||
著者所属(英) | ||||||
en | ||||||
Kanazawa University Graduate School of Medical Science | ||||||
著者所属(英) | ||||||
en | ||||||
Kanazawa University Institute of Nature and Environmental Technology | ||||||
著者所属(英) | ||||||
en | ||||||
Tokyo Medical and Dental University Graduate School | ||||||
著者所属(英) | ||||||
en | ||||||
Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences | ||||||
著者所属(英) | ||||||
en | ||||||
Waseda University Faculty of Education and Integral Arts and Science | ||||||
著者所属(英) | ||||||
en | ||||||
University of Toyama Graduate School of Medicine and Pharmaceutical Sciences | ||||||
著者所属(英) | ||||||
en | ||||||
University of Toyama Graduate School of Science and Engineering | ||||||
出版者 | ||||||
出版者 | 宇宙航空研究開発機構宇宙科学研究本部 | |||||
出版者(英) | ||||||
出版者 | Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS) | |||||
書誌情報 |
宇宙利用シンポジウム 第24回 平成19年度 en : Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium p. 230-233, 発行日 2008-03 |
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抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammalian bone. Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoblasts and osteoclasts with AlkaLine Phosphatase (ALP) and Tartrate-Resistant Acid Phosphatase (TRAP) as the respective markers and analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we indicated that osteoblastic and osteoclastic activities in the scale responded to the simulated microgravity with a three-dimensional clinostat. As these activities in the scale did not change by the stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in the scale osteoblasts and osteoclasts is a specific phenomenon. In addition, we analyzed the bone metabolism under 2-, 4-, and 7-gravity (G) loading with a centrifuge and compared them with the control (1-G). The osteoblastic activity significantly increased under 2- and 4-G loading. This activity increased remarkably under 7-G loading. On the other hand, we found that the osteoclastic activity significantly decreased under 2-G loading. Under 4-G loading, there was no significant difference between G-loaded scales and control scales. The osteoclastic activity tended to increase under 7-G loading. These results were similar to our previous study of acceleration by vibration. With the goal of developing a drug for bone diseases, the effect of novel bromomelatonin derivatives on osteoblasts and osteoclasts was examined. All bromomelatonin derivatives had an inhibitory action on osteoclasts. In particular, 1-benzyl-2, 4, 6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action. In addition, estrogen receptor mRNA (messenger Ribonucleic Acid) expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(exp -7) M)-treated scales. Therefore, this chemical will be useful to treat bone diseases, such as those experienced in space flight. | |||||
資料番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 資料番号: AA0063706059 |