Kagoshima University Graduate School of Medical and Dental Sciences
Kagoshima University Graduate School of Medical and Dental Sciences
Kagoshima University Graduate School of Medical and Dental Sciences
Kagoshima University Graduate School of Medical and Dental Sciences
Japan Space Forum Space Utilization Promotion Department
Japan Space Forum Space Utilization Promotion Department
Japan Aerospace Exploration Agency Institute of Space and Astronautical Science
Japan Aerospace Exploration Agency Institute of Space and Astronautical Science
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者（英）
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
雑誌名
宇宙利用シンポジウム 第24回 平成19年度
雑誌名(英)
Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium
ページ
331 - 332
発行年
2008-03
抄録（英）
Long term manned space missions of International Space Station (ISS) is in progress, and a plan to explore Moon as well as to visit the Mars is also underway. In space environment, 0.2 to 1 mSv a day of space radiation exposure and micro gravity environment will affect body, tissue and cellular metabolisms. In this study, the effects of 'Low-dose Irradiation' and 'Micro gravity' on total gene expressions change were tested using two human neuron-like cell lines, NB-1 and SK-N-SH. The cells were treated with (1) 0.1 mGy X-irradiation, or (2) micro gravity environment for 24 hr, or (3) with 0.1 mGy X-irradiation and the microgravity for 24 hr. After the treatments, mRNA (messenger Ribonucleic Acid) was isolated from the cells and DNA (DeoxyriboNucleic Acid) expression was studied with DNA microarray analysis. The results showed that the gene categories with intensive expression change in all irradiated or microgravity exposed conditions were 'mitochondrial genome' (8/13; 61.5 percent) and 'ribosomal protein' (70/184; 38.0 percent), and the two were downregulated in every condition. The results also showed that up-regulated specific gene was only one in every condition, that was 'Small Nuclear RNA Activating Complex, Polypetide 5; SNAPC5, which activate RNA transcription. The individual genes that appeared intensively downregulated in every condition were 'forkhead box m1 (foxm1)', a gene for oxidative stress related transcription factor, 'HSPA8', a constitutive expressed heat shock protein 'ALDOB', a glycolysis gene. These results suggest that the space low-dose irradiation and micro gravity condition decrease newly production of protein, and may affect mitochondrial bio-energy production ability, and may induce intracellular oxidative stress. The results also suggest that 0.1 mGy irradiation and microgravity condition may decrease cellular anti-stress ability.
宇宙線および微小重力による細胞内酸化ストレスの誘発
63706093.pdf
(233.09KB)
