Japan Aerospace Exploration Agency Institute of Space and Astronautical Science
Japan Aerospace Exploration Agency Space Environment Utilization Center
Tohoku University Graduate School of Life Sciences
Japan Aerospace Exploration Agency Institute of Space and Astronautical Science
出版者
宇宙航空研究開発機構宇宙科学研究本部
出版者(英)
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)
雑誌名
宇宙利用シンポジウム 第21回 平成16年度
雑誌名(英)
Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium
ページ
305 - 308
発行年
2005-03
抄録(英)
Gravity exerts a profound influence on plant growth. Proteomics may be the most promising technique to identify the proteins that are induced, repressed, or post-transcriptionally modified during gravity response in plants. In this research, the proteins controlled by gravity condition were identified. First, total protein extracted from Arabidopsis seedlings grown in 1 G-stationary and clino-rotated condition for seven days was used. Then, gravity-regulated proteins expression was analyzed by two-dimensional gel electrophoresis (2-DE). Several proteins which appeared different expressions between stationary and clino-rotated conditions were identified by Matrix Assisted Laser Desorption Ionization - Time Of Flight Mass Spectrometry (MALDI-TOFMS) analysis and Peptide Mass Fingerprinting (PMF).