WEKO3
アイテム
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微小重力応答プロテアーゼの検索
https://jaxa.repo.nii.ac.jp/records/13908
https://jaxa.repo.nii.ac.jp/records/13908308b0dfe-5f68-49a3-8fdf-a019961f6d7a
Item type | 会議発表論文 / Conference Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-03-26 | |||||
タイトル | ||||||
タイトル | 微小重力応答プロテアーゼの検索 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 微小重力 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | プロテアーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 紫外線照射 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ストレス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 落下塔 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ヒト細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | クロマトグラフィ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 親和力 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | microgravity | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | protease | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ultraviolet irradiation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | stress | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | drop tower | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | human cell | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | chromatography | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | affinity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
資源タイプ | conference paper | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
その他のタイトル(英) | ||||||
その他のタイトル | Search for the proteases which response under microgravity conditions | |||||
著者 |
唐田, 清伸
× 唐田, 清伸× 菅谷, 茂× 喜多, 和子× 鈴木, 信夫× Karata, Kiyonobu× Sugaya, Shigeru× Kita, Kazuko× Suzuki, Nobuo |
|||||
著者所属 | ||||||
千葉大学 大学院医学研究院 | ||||||
著者所属 | ||||||
千葉大学 大学院医学研究院 | ||||||
著者所属 | ||||||
千葉大学 大学院医学研究院 | ||||||
著者所属 | ||||||
千葉大学 大学院医学研究院 | ||||||
著者所属(英) | ||||||
en | ||||||
Chiba University Graduate School of Medicine | ||||||
著者所属(英) | ||||||
en | ||||||
Chiba University Graduate School of Medicine | ||||||
著者所属(英) | ||||||
en | ||||||
Chiba University Graduate School of Medicine | ||||||
著者所属(英) | ||||||
en | ||||||
Chiba University Graduate School of Medicine | ||||||
出版者 | ||||||
出版者 | 宇宙科学研究所 | |||||
出版者(英) | ||||||
出版者 | The Institute of Space and Astronautical Science (ISAS) | |||||
書誌情報 |
宇宙利用シンポジウム 第19回 平成14年度 en : Space Utilization Research: Proceedings of the Nineteenth Space Utilization Symposium p. 1-2, 発行日 2003-02 |
|||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | It was previously found that protease activation just after UV irradiation is induced in cultured human UVAP-1 cells. It was here investigated whether UV-activated protease activity is affected by exposure of human UVAP-1 cells to gravity-changing stress prior to UV irradiation. Levels of UV-induced protease activity were found to be increased to more extent in UVAP-1 cells exposed to drop shaft prior to UV irradiation than in cells exposed to UV irradiation alone. The cell lysate that was exposed to mock-treatment, gravity-changing stress alone, UV irradiation alone and gravity-changing stress plus UV irradiation, was partially purified by TSKgel G2000SW column chromatography. Some fractions that show high protease activity were detected in separated samples treated with both gravity-changing stress and UV irradiation. Protease activities of the fractions were effectively inhibited by cysteine protease inhibitor, E-64. To purify E-64 sensitive protease, E-64 affinity column chromatography was carried out using the cell lysate treated with both of gravity-changing stress and UV irradiation. The identification and analysis of purified proteases are in progress. | |||||
資料番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 資料番号: AA0045438001 |