State University of New York Dept. of Biochemistry and Cell Biology
State University of New York Dept. of Biochemistry and Cell Biology
State University of New York Dept. of Biochemistry and Cell Biology
著者所属（英）
State University of New York Department of Biochemistry and Cell Biology
State University of New York Department of Biochemistry and Cell Biology
State University of New York Department of Biochemistry and Cell Biology
出版者
宇宙開発事業団
出版者（英）
National Space Development Agency of Japan (NASDA)
IML-2 carried an experiment to evaluate cell division and somatic embryo progression in space. Test materials comprised embryogenic cells of daylily (Hemerocallis), a system which has been well-characterized in this laboratory from a developmental cell biology, physiology and chromosome structure perspective. A modest test (sample size = two 'petri dishes') on an earlier spaceflight (STS-57, J Mission) using the same system suggests the justification in using embryogenic cells of daylily as a model system for the study of space effects on cell division and development and chromosome structure in in vitro cultured cells in space. Cells with double nuclei and breaks were found in space samples but not in controls. Embryogenic initials are distributed on a membrane, placed on activated charcoal impregnated filter paper which is placed on a semi-solid nutrient medium held in place by a waffle-like support in a metal Petri-dish. Twelve dishes were flown in the IML-2 mission. Six dishes were designed to allow fixation in space. Half (3) were fixed on flight day 12. The other dishes served as controls. Flight samples showed chromosomal damage whereas ground controls did not. Furthermore, epidermal development of flight samples was considerably poorer than ground controls. Materials fixed in space were adequate for study but quality of fixation achieved in flight was poorer than that obtained post-flight. Nevertheless, the results show that perturbation is real and not an artifact of reentry or post-flight adaptation etc. No 1-G controls were run in Space. Hence the 'effect of micro-G on developing somatic embryos' was not spoken of but the effect of space flight factors.
Gravity and the stability of the differentiated state of plant somatic embryos (Pembsis)
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