LET, linear energy transfer cosmic radiation, RRMD, realtime radiation monitoring device, radiation dosimeter, Escherichia coli, plasmid, IML 2, 2nd international microgravity laboratory mission project, space experiment, microgravity, effect on living organisms, DNA, Escherichia coli mutant, mutation
その他のタイトル（英）
Dosimetry measurement of heavy particle beam in a space shuttle and investigation of its effect on living organisms (Radiation): Effect on Escherichia coli and plasmid DNA
National Space Development Agency of Japan Space Experiment Group
PL Gakuen Women's Junior College PL Botanical Institute
PL Gakuen Women's Junior College PL Botanical Institute
PL Gakuen Women's Junior College PL Botanical Institute
Japan Atomic Energy Research Institute Takasaki Radiation Chemistry Research Establishment
Japan Atomic Energy Research Institute Takasaki Radiation Chemistry Research Establishment
Nara Medical University
出版者
宇宙開発事業団
出版者（英）
National Space Development Agency of Japan (NASDA)
A space experiment conducted during the 2nd International Microgravity Laboratory Mission (IML-2) project was participated in. This study was designed to investigate the effect of high LET (Linear Energy Transfer) cosmic radiations on living organisms by loading the 'Realtime Radiation Monitoring Device (RRMD)' and 'Radiation Dosimeter' with biological samples, and performing post-flight analysis. As one of preparing procedure of biological samples, the preliminary experiment was performed on earth using Escherichia coli irradiated with Ultraviolet (UV) light. After preparing the biological samples, which were dried E. coli DNA (Deoxyribonucleic Acid) repair-deficient mutants and the shuttle vector plasmid pZ189 DNA, they were placed in the Dosimeters and in the RRMD, which was a Biospecimen Box sandwiched between 'Harzlas' plastic radiation detectors. These were then loaded into the space shuttle 'Columbia' while the identical box and dosimeters were let at the NASA John F. Kennedy Space Center (KSC) as a control on earth. 'Columbia (flight No. STS-65)' was launched from KSC in Florida, USA on July 9, 1994, and landing site was the same place as launch. Mission duration was 14 days. After the return of 'Columbia' to earth, the following two subjects were studied; (1) the lethality and mutagenicity of high LET cosmic radiation on E. coli mutants, and (2) the relation between high LET cosmic radiations and the mutation of pZ189 DNA. For E. coli KMBL3835 (wild type), KY383 (lexA(sup -)), KY385 (recA(sup -)) and KY386 (uvrA(sup -)), there was little difference between the cell viabilities of the space samples and those of the ground samples. Likewise, there was little difference between space and ground in the ratios of mutation frequency of the E. coli mutants. Furthermore, the survival and mutation frequency of supF gene of pZ189 DNA space samples did not differ from those of the ground samples.
宇宙船内における重粒子線による線量計測とその生物効果実験(RADIATION):大腸菌細胞とプラスミドDNAについて
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