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アイテム
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Test materials comprised embryogenic cells of daylily (Hemerocallis), a system which has been well-characterized in this laboratory from a developmental cell biology, physiology and chromosome structure perspective. A modest test (sample size = two \u0027petri dishes\u0027) on an earlier spaceflight (STS-57, J Mission) using the same system suggests the justification in using embryogenic cells of daylily as a model system for the study of space effects on cell division and development and chromosome structure in in vitro cultured cells in space. Cells with double nuclei and breaks were found in space samples but not in controls. Embryogenic initials are distributed on a membrane, placed on activated charcoal impregnated filter paper which is placed on a semi-solid nutrient medium held in place by a waffle-like support in a metal Petri-dish. Twelve dishes were flown in the IML-2 mission. Six dishes were designed to allow fixation in space. Half (3) were fixed on flight day 12. The other dishes served as controls. Flight samples showed chromosomal damage whereas ground controls did not. Furthermore, epidermal development of flight samples was considerably poorer than ground controls. Materials fixed in space were adequate for study but quality of fixation achieved in flight was poorer than that obtained post-flight. Nevertheless, the results show that perturbation is real and not an artifact of reentry or post-flight adaptation etc. No 1-G controls were run in Space. Hence the \u0027effect of micro-G on developing somatic embryos\u0027 was not spoken of but the effect of space flight factors.", "subitem_description_type": "Other"}]}, "item_3_description_32": {"attribute_name": "資料番号", "attribute_value_mlt": [{"subitem_description": "資料番号: AA0000271007", "subitem_description_type": "Other"}]}, "item_3_description_33": {"attribute_name": "レポート番号", "attribute_value_mlt": [{"subitem_description": "レポート番号: NASDA-TMR-960004", "subitem_description_type": "Other"}]}, "item_3_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "宇宙開発事業団"}]}, "item_3_publisher_9": {"attribute_name": "出版者(英)", "attribute_value_mlt": [{"subitem_publisher": "National Space Development Agency of Japan (NASDA)"}]}, "item_3_source_id_21": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1345-7888", "subitem_source_identifier_type": "ISSN"}]}, "item_3_source_id_24": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00364784", "subitem_source_identifier_type": "NCID"}]}, "item_3_text_35": {"attribute_name": "JAXAカテゴリ", "attribute_value_mlt": [{"subitem_text_value": "JAXAカテゴリ: 宇宙開発事業団"}]}, "item_3_text_43": {"attribute_name": "DSpaceコレクション番号", "attribute_value_mlt": [{"subitem_text_value": "DSpaceコレクション番号: 7"}]}, "item_3_text_6": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "State University of New York Dept. of Biochemistry and Cell Biology"}, {"subitem_text_value": "State University of New York Dept. of Biochemistry and Cell Biology"}, {"subitem_text_value": "State University of New York Dept. of Biochemistry and Cell Biology"}]}, "item_3_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "State University of New York Department of Biochemistry and Cell Biology"}, {"subitem_text_language": "en", "subitem_text_value": "State University of New York Department of Biochemistry and Cell Biology"}, {"subitem_text_language": "en", "subitem_text_value": "State University of New York Department of Biochemistry and Cell Biology"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Krikorian, A. 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Gravity and the stability of the differentiated state of plant somatic embryos (Pembsis)
https://jaxa.repo.nii.ac.jp/records/39716
https://jaxa.repo.nii.ac.jp/records/3971690b74209-a846-4461-a2b5-3b6b88b463eb
名前 / ファイル | ライセンス | アクション |
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00271007.pdf (1.1 MB)
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Item type | テクニカルレポート / Technical Report(1) | |||||
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公開日 | 2015-03-26 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Gravity and the stability of the differentiated state of plant somatic embryos (Pembsis) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 体細胞胚 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 細胞分裂 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | カンゾウ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 試験管内 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 培養細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ペトリ皿 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 固定 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 染色体損傷 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 宇宙実験 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 微小重力 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | somatic embryo | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cell division | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | daylily | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | in vitro | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cultured cell | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Petri dish | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | fixation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | chromosomal damage | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | space experiment | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | microgravity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18gh | |||||
資源タイプ | technical report | |||||
その他のタイトル | ||||||
その他のタイトル | 重力と植物体細胞胚の分化状態の安定性について(PEMBSIS) | |||||
著者 |
Krikorian, A. D.
× Krikorian, A. D.× OConnor, Stefania A.× Kann, R. P. |
|||||
著者所属 | ||||||
State University of New York Dept. of Biochemistry and Cell Biology | ||||||
著者所属 | ||||||
State University of New York Dept. of Biochemistry and Cell Biology | ||||||
著者所属 | ||||||
State University of New York Dept. of Biochemistry and Cell Biology | ||||||
著者所属(英) | ||||||
en | ||||||
State University of New York Department of Biochemistry and Cell Biology | ||||||
著者所属(英) | ||||||
en | ||||||
State University of New York Department of Biochemistry and Cell Biology | ||||||
著者所属(英) | ||||||
en | ||||||
State University of New York Department of Biochemistry and Cell Biology | ||||||
出版者 | ||||||
出版者 | 宇宙開発事業団 | |||||
出版者(英) | ||||||
出版者 | National Space Development Agency of Japan (NASDA) | |||||
書誌情報 |
宇宙開発事業団技術報告 en : NASDA Technical Memorandum p. 163-178, 発行日 1996-03-15 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | IML-2では、宇宙での細胞分裂と体細胞胚発達を検討評価するための実験をおこなった。試験材料はカンゾウ(Hemerocallis)の胚細胞で、研究室では発生細胞生物学、生理学、染色体構造全体像から、この系の特徴を十分把握している。以前の宇宙フライト(STS-57J Mission)時の同一の系を使用した、限られた試験(試料サイズ=「ペトリ皿2枚」)では、宇宙での試験管内培養細胞において、細胞分裂、発生および染色体構造に及ぼす宇宙の影響を研究するモデル系として、カンゾウの胚細胞を使用することは妥当であることを示唆した。2倍核や破損した核のある細胞が宇宙試料では見つかったが、対照では見つからなかった。胚の未分化細胞を膜の上に蒔き、膜の下には活性炭の染み込んだ濾紙があり、その下には半個体状の培地が金属製ペトリ皿の中にワッフル状土台としておさまっている。IML-2ミッションでは、12皿を宇宙飛行させた。6皿は宇宙で固定するように計画した。3皿は飛行12日目に固定した。残りの皿は対照とした。フライト試料は染色体損傷を示したが、地上対照は示さなかった。さらに、フライト試料の上皮組織の発生は、地上対照より相当遅れていた。宇宙で固定した材料は、研究には十分なものであるが、フライト時におこなわれた固定の質は、フライト後に得られたものの質よりも劣っていた。にもかかわらず、混乱状態は実在するもので、再突入の人為的結果やフライト後の適応などではないことを結果は示している。宇宙で1G対照はおこなわれていない。従って、「発生中の体細胞胚に対するミクロ-Gの影響」ではなく「宇宙飛行要因の影響」について論ずることになる。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | IML-2 carried an experiment to evaluate cell division and somatic embryo progression in space. Test materials comprised embryogenic cells of daylily (Hemerocallis), a system which has been well-characterized in this laboratory from a developmental cell biology, physiology and chromosome structure perspective. A modest test (sample size = two 'petri dishes') on an earlier spaceflight (STS-57, J Mission) using the same system suggests the justification in using embryogenic cells of daylily as a model system for the study of space effects on cell division and development and chromosome structure in in vitro cultured cells in space. Cells with double nuclei and breaks were found in space samples but not in controls. Embryogenic initials are distributed on a membrane, placed on activated charcoal impregnated filter paper which is placed on a semi-solid nutrient medium held in place by a waffle-like support in a metal Petri-dish. Twelve dishes were flown in the IML-2 mission. Six dishes were designed to allow fixation in space. Half (3) were fixed on flight day 12. The other dishes served as controls. Flight samples showed chromosomal damage whereas ground controls did not. Furthermore, epidermal development of flight samples was considerably poorer than ground controls. Materials fixed in space were adequate for study but quality of fixation achieved in flight was poorer than that obtained post-flight. Nevertheless, the results show that perturbation is real and not an artifact of reentry or post-flight adaptation etc. No 1-G controls were run in Space. Hence the 'effect of micro-G on developing somatic embryos' was not spoken of but the effect of space flight factors. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1345-7888 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00364784 | |||||
資料番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 資料番号: AA0000271007 | |||||
レポート番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | レポート番号: NASDA-TMR-960004 |