@inproceedings{oai:jaxa.repo.nii.ac.jp:00013101,
 author = {谷田貝, 文夫 and 戎崎, 俊一 and 本間, 正充 and 石岡, 憲昭 and Yatagai, Fumio and Ebisuzaki, Tosikazu and Honma, Masamitsu and Ishioka, Noriaki},
 book = {宇宙利用シンポジウム: 第27回: 平成22年度, Space Utilization Research, Vol. 27 2011: Proceedings of The Twenty-seventh Space Utilization Symposium},
 month = {Mar},
 note = {第27回宇宙利用シンポジウム (2011年1月24日-25日, 宇宙航空研究開発機構宇宙科学研究所相模原キャンパス), 相模原市, 神奈川県, The Twenty-seventh Space Utilization Symposium (January 24-25, 2011. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan, An experiment was successfully conducted at "Kibo" module of International Space Station (ISS) from November 14, 2008 to March 28, 2009. In order to estimate the space-radiation effects, which were not influenced by other space environmental factors such as microgravity, frozen cells of human lymphoblastoid TK6 were brought to "Kibo" module and preserved there for134 days (total dose of 72 mSv). After recovery to Earth, we observed a ca.2.3-fold induction in thymidine kinase (TK) deficient mutation over the ground control. The indirect effects of space radiation were also detected by using the same frozen cell sample following the post-flight treatments such as 2 Gy X-ray irradiation or I-SceI digestion. The TK- mutation frequency after the X-ray irradiation was found to be ca.50% of the level of the X-ray irradiated ground control. The repair-efficiency for DNA double-strand break (I-sceI digestion) was ca.2-fold higher than the ground control case. These results suggest the possibility that frozen cells can record DNA damage accumulated during space flight and subsequently express the genetic and cellular responses when grown on earth. A part of the frozen cells were incubated under microG or 1 G in "Kibo" for 8 days. The post-flight assays demonstrated that cell-viability and mutability after the microG incubation were reduced to 55% and 59% of the level of 1G incubation, respectively. These results might suggest the possibility of microgravity influence in DNA repair, but future experiments are required for confirmation. An idea for the future experiment is also described here., 形態: カラー図版あり, Physical characteristics: Original contains color illustrations, 資料番号: AA0065129041},
 pages = {111--112},
 publisher = {宇宙航空研究開発機構宇宙科学研究所 (JAXA)(ISAS), Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)},
 title = {きぼう棟利用実験の成果と今後の展望: 宇宙放射線の生物影響},
 volume = {27},
 year = {2011}
}