@inproceedings{oai:jaxa.repo.nii.ac.jp:00013131,
 author = {清本, 正人 and 黒谷, 明美 and 江口, 星雄 and 山口, 守 and Kiyomoto, Masato and Izumi-Kurotani, Akemi and Eguchi, Hoshio and Yamaguchi, Mamoru},
 book = {宇宙利用シンポジウム: 第27回: 平成22年度, Space Utilization Research, Vol. 27 2011: Proceedings of The Twenty-seventh Space Utilization Symposium},
 month = {Mar},
 note = {第27回宇宙利用シンポジウム (2011年1月24日-25日, 宇宙航空研究開発機構宇宙科学研究所相模原キャンパス), 相模原市, 神奈川県, The Twenty-seventh Space Utilization Symposium (January 24-25, 2011. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan, Sea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicules grow up to larval skeletons. The skeletogenic cells are called primary mesenchyme cells derived from micromeres at 16-cell stage. We already reported a promotive effect of hypergravity on skeletogenesis in the culture of the skeletogenic cells with low concentration of horse serum. For the culture of skeletogenic cells, micromeres isolated by sucrose gradient are used. In this study, we examined more simple method to isolate skeletogenic cells. In calcium deficient seawater, embryos developed without cell adhesion and failed to form blastura. At the time of hatching, most cells were dispersed but mesenchyme cells attached on the substrate. By the gentle stream, dissociated cells were removed and mesenchyme cells left on the bottom were used for the culture. These mesenchyme sometimes contained not only skeletogenic cells but also secondary mesenchyme cells. This isolation method is simple and possibly is useful to improve a procedure of the gravity experiment., 形態: カラー図版あり, Physical characteristics: Original contains color illustrations, 資料番号: AA0065129071},
 pages = {193--194},
 publisher = {宇宙航空研究開発機構宇宙科学研究所 (JAXA)(ISAS), Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)},
 title = {重力実験のためのウニの幼生骨格系間充織の培養の検討},
 volume = {27},
 year = {2011}
}