{"created":"2023-06-20T14:45:40.532051+00:00","id":13131,"links":{},"metadata":{"_buckets":{"deposit":"1e4a536b-af51-4eea-8299-df032cbf92a8"},"_deposit":{"created_by":1,"id":"13131","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13131"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013131","sets":["1543:1633:1640","1887:1891"]},"author_link":["117650","117654","117652","117655","117651","117656","117657","117653"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"The culture condition of larval skeletogenic mesenchyme cell for the gravity experiment"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"194","bibliographicPageStart":"193","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム: 第27回: 平成22年度"},{"bibliographic_title":"Space Utilization Research, Vol. 27 2011: Proceedings of The Twenty-seventh Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_14":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第27回宇宙利用シンポジウム (2011年1月24日-25日, 宇宙航空研究開発機構宇宙科学研究所相模原キャンパス), 相模原市, 神奈川県","subitem_description_type":"Other"}]},"item_5_description_15":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）（英）","attribute_value_mlt":[{"subitem_description":"The Twenty-seventh Space Utilization Symposium (January 24-25, 2011. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan","subitem_description_type":"Other"}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Sea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicules grow up to larval skeletons. The skeletogenic cells are called primary mesenchyme cells derived from micromeres at 16-cell stage. We already reported a promotive effect of hypergravity on skeletogenesis in the culture of the skeletogenic cells with low concentration of horse serum. For the culture of skeletogenic cells, micromeres isolated by sucrose gradient are used. In this study, we examined more simple method to isolate skeletogenic cells. In calcium deficient seawater, embryos developed without cell adhesion and failed to form blastura. At the time of hatching, most cells were dispersed but mesenchyme cells attached on the substrate. By the gentle stream, dissociated cells were removed and mesenchyme cells left on the bottom were used for the culture. These mesenchyme sometimes contained not only skeletogenic cells but also secondary mesenchyme cells. This isolation method is simple and possibly is useful to improve a procedure of the gravity experiment.","subitem_description_type":"Other"}]},"item_5_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"}]},"item_5_description_19":{"attribute_name":"内容記述（英）","attribute_value_mlt":[{"subitem_description":"Physical characteristics: Original contains color illustrations","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0065129071","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究所 (JAXA)(ISAS)"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)"}]},"item_5_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10324210","subitem_source_identifier_type":"NCID"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"お茶の水大学"},{"subitem_text_value":"宇宙航空研究開発機構宇宙科学研究所 (JAXA) (ISAS)"},{"subitem_text_value":"東京大学"},{"subitem_text_value":"お茶の水大学"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Ochanomizu University"},{"subitem_text_language":"en","subitem_text_value":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA) (ISAS)"},{"subitem_text_language":"en","subitem_text_value":"University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Ochanomizu University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"清本, 正人"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒谷, 明美"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"江口, 星雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 守"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiyomoto, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumi-Kurotani, Akemi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Hoshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Mamoru","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"65129071.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"65129071.pdf","url":"https://jaxa.repo.nii.ac.jp/record/13131/files/65129071.pdf"},"version_id":"e9c4b585-c4c5-4631-8507-0d9c5e062262"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"skeletogenic cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sea urchin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"horse serum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"calcium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hypergravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"重力実験のためのウニの幼生骨格系間充織の培養の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"重力実験のためのウニの幼生骨格系間充織の培養の検討"}]},"item_type_id":"5","owner":"1","path":["1640","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13131","relation_version_is_last":true,"title":["重力実験のためのウニの幼生骨格系間充織の培養の検討"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T06:05:18.842536+00:00"}