@inproceedings{oai:jaxa.repo.nii.ac.jp:00013143, author = {山口, 進康 and 稗田, はつき and 一條, 知昭 and 那須, 正夫 and Yamaguchi, Nobuyasu and Hieda, Hatsuki and Ichijo, Tomoaki and Nasu, Masao}, book = {宇宙利用シンポジウム: 第27回: 平成22年度, Space Utilization Research, Vol. 27 2011: Proceedings of The Twenty-seventh Space Utilization Symposium}, month = {Mar}, note = {第27回宇宙利用シンポジウム (2011年1月24日-25日, 宇宙航空研究開発機構宇宙科学研究所相模原キャンパス), 相模原市, 神奈川県, The Twenty-seventh Space Utilization Symposium (January 24-25, 2011. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan, Microbes exist everywhere, even in a space habitat. The microbial ecosystem may differ from those on Earth in such a closed environment under microgravity. It is necessary to investigate the relationship between humans and microbes, and how microbes influence the systems and materials in this environment. The objective of our experiment is to monitor the abundance of bacteria and predict their dynamics in "KIBO", the Japanese experiment module of the International Space Station (ISS), from our own viewpoints. This time, we measured the bacterial abundance on a multi protocol converter (MPC) used in the ISS. Bacterial cells on the surfaces and parts outside and inside of a MPC were collected with swab or "microbecollecting adhesive sheet (Yamaguchi, et al. J. Microbial. Methods, 53: 405, 2003)", and bacterial number was measured by quantitative realtime PCR targeting bacterial 16S rRNA gene sequences following DNAextraction from bacterial cells collected with swab or sampling sheet. Sampling method with the adhesive sheet is simple, rapid, and reproducible. This sheet does not require water to recover the bacteria from the targeted surface, while the swab method needs water to moisturize the head. This sampling sheet is suitable to collect bacterial cells on solid surfaces and we believe this sheet would be useful device in the field of astrobiology., 形態: カラー図版あり, Physical characteristics: Original contains color illustrations, 資料番号: AA0065129083}, pages = {221--223}, publisher = {宇宙航空研究開発機構宇宙科学研究所 (JAXA)(ISAS), Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)}, title = {国際宇宙ステーションから回収した機器における細菌の現存量}, volume = {27}, year = {2011} }