@inproceedings{oai:jaxa.repo.nii.ac.jp:00013200, author = {増野, 陽一 and 中井, 亮佑 and 中村, 慶子 and 幸村, 基世 and 渋谷, 絵里 and 右山, 絵理 and 黒田, 麻美 and 高橋, 寛 and 長沼, 毅 and 伊村, 智 and 岩月, 輝希 and 金子, 竹男 and 小林, 克己 and 小林, 憲正 and 佐藤, 皓 and 三枝, 誠行 and 嶋田, 和人 and 白壁, 義久 and 高野, 淑識 and 高山, 健 and 俵, 裕子 and 橋本, 博文 and 森田, 洋平 and 保田, 浩志 and 山下, 雅道 and Mashino, Yoichi and Nakai, Ryosuke and Nakamura, Keiko and Yukimura, Kiyo and Shibuya, Eri and Migiyama, Eri and Kuroda, A. and Takahashi, H. and Naganuma, Takeshi and Satoshi, Imura and Iwatsuki, T. and Kaneko, T. and Kobayashi, K. and Kobayashi, K. and Sato, H. and Saigusa, Masayuki and Shimada, Kazuhito and Shirakabe, Yoshihisa and Takano, Yoshinori and Takayama, Ken and Tawara, Hiroko and Hashimoto, Hirofumi and Morita, Yohei and Yasuda, Hiroshi and Yamashita, Masamichi}, book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium}, month = {Feb}, note = {第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan), One of major problems in astrobiology is intense radiation, theoretical and experimental studies on biological protection and tolerance to radiation are needed. Wet and freeze-dried microbial cells were irradiated with high-dose X-ray for 0 to 10,000 Gy and high-energy Fe ions for 0 to 4,850 Gy to examine their survivability based on the most probably number (MPN), the biological "energy currency", i.e., adenosine 5'-triphosphate (ATP) and genomic DNA fragmentation. The well known radiation-resistant bacteria such as genus Deinococcus radiodurans, highly stress-resistant spore former Bacillus subtilis spore and radiation-sensitive Escherichia coli were used for this experiment. Irradiation of synchrotron X-ray (0.2 nm) at a high dose rate of 4.4 Gy/s (500 R/s) was done at KEK Photon Factory. Freeze dried D. raduidurans and E. coli cells showed higher survivability than their wet cells. But B. subtilis spores have not changed their survivability wet or freeze-dried. Freeze-dried celluar ATP showed no decrease against to the dose. In addition, genomic DNA fragmentation was not easy to generated in freeze-dried cells. Irradiation of 500 MeV Fe ion at a dose rate of 0.172 Gy/s (2.5×10(exp8) particles/s) was done at NIRS HIMAC. Generally similar results to X-ray irradiation were observed, but ATP showed exponential decrease in wet cells against to the dose., 形態: カラー図版あり, 共催: 日本学術会議, 著者人数: 25人, Physical characteristics: Original contains color illustrations, joint hosting: The Science Council of Japan, 資料番号: AA0064730053}, publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)}, title = {大線量エックス線および高エネルギーFeイオン照射に対する微生物}, volume = {26}, year = {2010} }