@inproceedings{oai:jaxa.repo.nii.ac.jp:00013214, author = {神阪, 盛一郎 and 保尊, 隆享 and 若林, 和幸 and 曽我, 康一 and 笠原, 宏一 and 山田, 晃弘 and 高橋, 秀幸 and 田坂, 昌生 and 西谷, 一彦 and 上田, 純一 and 宮本, 健助 and 山田, 恭司 and 増田, 恭次郎 and 若杉, 達也 and 唐原, 一郎 and 酒井, 英男 and 田山, 一郎 and 嶋津, 徹 and 矢野, 幸子 and 石岡, 憲昭 and 北宅, 善昭 and 多胡, 靖宏 and 後藤, 英司 and 久米, 篤 and 笠原, 春夫 and 山口, 和男 and 西内, 巧 and 鎌田, 源司 and 山下, 雅道 and Kamisaka, Seiichiro and Hoson, Takayuki and Wakabayashi, Kazuyuki and Soga, Kouichi and Kasahara, Hirokazu and Yamada, Mitsuhiro and Takahashi, Hideyuki and Tasaka, Masao and Nishitani, Kazuhiko and Ueda, Junichi and Miyamoto, Kensuke and Yamada, Kyoji and Masuda, Kyojiro and Wakasugi, Tatsuya and Karahara, Karahara Ichirou and Sakai, Hideo and Tayama, Ichiro and Shimazu, Toru and Yano, Sachiko and Ishioka, Noriaki and Kitaya, Yoshiaki and Tako, Yasuhiro and Goto, Eiji and Kume, Atsushi and Kasahara, Haruo and Yamaguchi, Kazuo and Nishiuchi, Takumi and Kamada, Motoshi and Yamashita, Masamichi}, book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium}, month = {Feb}, note = {第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan), Plant life cycle consists of successive phases of vegetative and reproductive growth. The effects of altered gravity conditions on reproductive growth are not understood well compared to vegetative growth. We have performed proteome analysis to examine the effect of hypergravity on reproductive growth of Arabidopsis (Arabidopsis thaliana (L.) Heynh. ecotype Columbia-0). Plants grown for 20-26 days under were exposed to hypergravity at 300 x g or placed at 1 x g for 24 h in the dark. Proteins extracted from the flower buds with a buffer containing Triton X-100 were subjected to two-dimensional electrophoresis. The polypeptides, that were up-regulated or down-regulated by hypergravity, were analyzed by matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry, to compare the present proteome results with our previous transcriptome results. Proteome analysis showed that hypergravity changed the expression of proteins involved in protein folding and translocation, ethylene synthesis and stress response., 共催: 日本学術会議, 著者人数: 29人, joint hosting: The Science Council of Japan, 資料番号: AA0064730067}, publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)}, title = {平成21年度WG「高等植物の生活環」活動報告}, volume = {26}, year = {2010} }