@inproceedings{oai:jaxa.repo.nii.ac.jp:00013217,
 author = {清本, 正人 and 黒谷, 明美 and 江口, 星雄 and 山口, 守 and Kiyomoto, Masato and Izumi-Kurotani, Akemi and Eguchi, Hoshio and Yamaguchi, Mamoru},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Feb},
 note = {第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan), Sea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicules grow up to larval skeletons. The skeletogenic cells are called primary mesenchyme cells derived from micromeres at 16-cell stage. We already reported a promotive effect of hypergravity on skeletogenesis in the culture of the skeletogenic cells with low concentration of horse serum. In this study, we examined the culture condition, the component necessary for spicule formation. The low activity of gel filtration fraction was recovered by the addition of affinity heparin column flow through fraction. VEGF, one candidate of the factor for spicule formation, was not effective for micromere culture, even with low concentration of horse serum. These results show the possibility that two or more factors are necessary for spicule formation in the culture of micromeres., 形態: カラー図版あり, 共催: 日本学術会議, Physical characteristics: Original contains color illustrations, joint hosting: The Science Council of Japan, 資料番号: AA0064730070},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {重力実験のためのウニの幼生骨格の培養系の検討},
 volume = {26},
 year = {2010}
}