@inproceedings{oai:jaxa.repo.nii.ac.jp:00013238,
 author = {高橋, 昭久 and 大西, 武雄 and 鈴木, ひろみ and 嶋津, 徹 and 関, 真也 and 橋爪, 藤子 and 大森, 克徳 and 石岡, 憲昭 and Takahashi, Akihisa and Onishi, Takeo and Suzuki, Hiromi and Shimazu, Toru and Seki, Masaya and Hashizume, Toko and Omori, Katsunori and Ishioka, Noriaki},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Feb},
 note = {第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan), The space environment contains two major biologically significant influences: space radiations and microgravity. A p53 tumor suppressor protein plays a role as a guardian of the genome through the activity of p53-centered signal transduction pathways. The aim of this study was to clarify the biological effects of space radiations, microgravity and a space environment on the gene and protein expression of p53-dependent regulated genes. Space experiments were performed with two human cultured lymphoblastoid cell lines: one cells line (TSCE5) bears a wild-type p53 gene status, and another cells line (WTK1) bears a mutated p53 gene status. Under one gravity or microgravity condition, the cells were grown in the cell biology experimental facility (CBEF) of the International Space Station (ISS) for 8 days without experiencing the stress during launching and landing because the cells were frozen during these periods. Ground control samples also were cultured for 8 days in the CBEF on the ground during the spaceflight. Gene and protein expression was analyzed by using DNA chip (a 44k whole human genome microarray, Agilent Technologies Inc.) and protein chip (PanoramaTM Ab MicroArray, Sigma-Aldrich Co.), respectively. We report the results and discussion from the viewpoint of the functions of the up-regulated and down-regulated genes after an exposure to space radiations and/or microgravity. The initial goal of this space experiment was completely achieved. It is expected that data from this type of work will be helpful in designing physical protection from the deleterious effects of sapace radiations during long term stays in space., 形態: カラー図版あり, 共催: 日本学術会議, Physical characteristics: Original contains color illustrations, joint hosting: The Science Council of Japan, 資料番号: AA0064730091},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {「きぼう」でのRad Gene 宇宙実験報告2: ヒト培養系における宇宙でのp53 依存性遺伝子およびタンパク質発現},
 volume = {26},
 year = {2010}
}