{"created":"2023-06-20T14:45:45.385885+00:00","id":13238,"links":{},"metadata":{"_buckets":{"deposit":"ee7cd6e0-5fe5-4e4f-8bd4-6912ce7da9d1"},"_deposit":{"created_by":1,"id":"13238","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13238"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013238","sets":["1543:1633:1641","1887:1891"]},"author_link":["119692","119694","119696","119686","119695","119687","119688","119691","119685","119693","119700","119699","119698","119690","119697","119689"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Space experiment \"Rad Gene\" at Kibo-report 2: p53-Dependent gene and protein expression in human cultured cells during spaceflight"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム"},{"bibliographic_title":"Space Utilization Research: Proceedings of Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_14":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス)","subitem_description_type":"Other"}]},"item_5_description_15":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）（英）","attribute_value_mlt":[{"subitem_description":"The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan)","subitem_description_type":"Other"}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The space environment contains two major biologically significant influences: space radiations and microgravity. A p53 tumor suppressor protein plays a role as a guardian of the genome through the activity of p53-centered signal transduction pathways. The aim of this study was to clarify the biological effects of space radiations, microgravity and a space environment on the gene and protein expression of p53-dependent regulated genes. Space experiments were performed with two human cultured lymphoblastoid cell lines: one cells line (TSCE5) bears a wild-type p53 gene status, and another cells line (WTK1) bears a mutated p53 gene status. Under one gravity or microgravity condition, the cells were grown in the cell biology experimental facility (CBEF) of the International Space Station (ISS) for 8 days without experiencing the stress during launching and landing because the cells were frozen during these periods. Ground control samples also were cultured for 8 days in the CBEF on the ground during the spaceflight. Gene and protein expression was analyzed by using DNA chip (a 44k whole human genome microarray, Agilent Technologies Inc.) and protein chip (PanoramaTM Ab MicroArray, Sigma-Aldrich Co.), respectively. We report the results and discussion from the viewpoint of the functions of the up-regulated and down-regulated genes after an exposure to space radiations and/or microgravity. The initial goal of this space experiment was completely achieved. It is expected that data from this type of work will be helpful in designing physical protection from the deleterious effects of sapace radiations during long term stays in space.","subitem_description_type":"Other"}]},"item_5_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"},{"subitem_description":"共催: 日本学術会議","subitem_description_type":"Other"}]},"item_5_description_19":{"attribute_name":"内容記述（英）","attribute_value_mlt":[{"subitem_description":"Physical characteristics: Original contains color illustrations","subitem_description_type":"Other"},{"subitem_description":"joint hosting: The Science Council of Japan","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0064730091","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)"}]},"item_5_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10324210","subitem_source_identifier_type":"NCID"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"奈良県立医科大学 : 宇宙航空研究開発機構"},{"subitem_text_value":"奈良県立医科大学 : 宇宙航空研究開発機構"},{"subitem_text_value":"宇宙航空研究開発機構 : 日本宇宙フォーラム宇宙利用事業部生命科学研究グループ"},{"subitem_text_value":"日本宇宙フォーラム"},{"subitem_text_value":"鹿児島大学 : エイ・イー・エス"},{"subitem_text_value":"鹿児島大学 : エイ・イー・エス"},{"subitem_text_value":"宇宙航空研究開発機構宇宙科学研究本部ISS科学プロジェクト室"},{"subitem_text_value":"宇宙航空研究開発機構宇宙科学研究本部ISS科学プロジェクト室 : 鹿児島大学"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nara Medical University : Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Nara Medical University : Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency (JAXA) : Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum"},{"subitem_text_language":"en","subitem_text_value":"Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum"},{"subitem_text_language":"en","subitem_text_value":"Kagoshima University : Advanced Engineering Services (AES)"},{"subitem_text_language":"en","subitem_text_value":"Kagoshima University : Advanced Engineering Services (AES)"},{"subitem_text_language":"en","subitem_text_value":"ISS Science Project Office, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)"},{"subitem_text_language":"en","subitem_text_value":"ISS Science Project Office, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS) : Kagoshima University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高橋, 昭久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大西, 武雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, ひろみ"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"嶋津, 徹"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"関, 真也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"橋爪, 藤子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大森, 克徳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石岡, 憲昭"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Akihisa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onishi, Takeo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Hiromi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimazu, Toru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seki, Masaya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashizume, Toko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omori, Katsunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishioka, Noriaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"64730091.pdf","filesize":[{"value":"564.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"64730091.pdf","url":"https://jaxa.repo.nii.ac.jp/record/13238/files/64730091.pdf"},"version_id":"5871a3a4-8e07-41e5-99f2-e62317439df7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"International Space Station, space radiations, microgravity, p53, gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"「きぼう」でのRad Gene 宇宙実験報告2: ヒト培養系における宇宙でのp53 依存性遺伝子およびタンパク質発現","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"「きぼう」でのRad Gene 宇宙実験報告2: ヒト培養系における宇宙でのp53 依存性遺伝子およびタンパク質発現"}]},"item_type_id":"5","owner":"1","path":["1641","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13238","relation_version_is_last":true,"title":["「きぼう」でのRad Gene 宇宙実験報告2: ヒト培養系における宇宙でのp53 依存性遺伝子およびタンパク質発現"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T06:01:56.257056+00:00"}