@inproceedings{oai:jaxa.repo.nii.ac.jp:00013242,
 author = {谷田貝, 文夫 and 榎本, 秀一 and 堂前, 直 and 本間, 正充 and 鵜飼, 明子 and 大森, 克徳 and 石岡, 憲昭 and Yatagai, Fumio and Enomoto, Shuichi and Domae, Naoshi and Honma, Masamitsu and Ukai, Akiko and Omori, Katsunori and Ishioka, Noriaki},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Feb},
 note = {第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan), An experiment was successfully conducted at'Kibo' module of International Space Station (ISS) from November 14, 2008 to March 28, 2009. In this research project, a focus was made on biological effects of high-radiation background among various factors of space environments. Human lymphoblastoid TK6 cells were frozen and sent to ISS and finally brought back to earth; mainly preserved as frozen in ISS and receiving a total 72 mSv. In addition, we tried to elucidate influences of microgravity on such radiation effects by incubating them at 37C under microG or 1G for 8 days and then refreezing them in ISS. The mutational assays for the non-incubated flight-cells demonstrated the following effects: i) ca.2.3-fold induction of thymidine kinase-deficient (TK(sup -)) mutations over the level of ground-control cell, ii) a suppression (ca.56%) of TK(sup -) mutations, induced by a challenging dose of 2 Gy X-rays and iii) a more efficient repair of DNA double-strand breaks created by I-SceI expression (ca.1.9- and ca.1.8-fold due to non-homologous end joining and homologous recombination, respectively). The characterization (LOH analysis) of the TK(sup -) mutants obtained in the above assays also supports the possibility that frozen cells can record damage accumulated during space travel and subsequently express the genetic and cellular responses when grown on earth. The preliminary results on the microgravity effects, estimated from the mutation induction in the incubated flight-cells, are briefly introduced here., 形態: カラー図版あり, 共催: 日本学術会議, Physical characteristics: Original contains color illustrations, joint hosting: The Science Council of Japan, 資料番号: AA0064730095},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {ISS実験: 突然変異の検出と適応応答の検証},
 volume = {26},
 year = {2010}
}