{"created":"2023-06-20T14:45:45.604775+00:00","id":13243,"links":{},"metadata":{"_buckets":{"deposit":"44f6df0c-73c1-4109-b91e-f30ee52c9721"},"_deposit":{"created_by":1,"id":"13243","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13243"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013243","sets":["1543:1633:1641","1887:1891"]},"author_link":["119768","119763","119751","119755","119756","119769","119752","119770","119771","119758","119767","119760","119765","119754","119772","119759","119766","119753","119761","119762","119757","119773","119764"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Space experiment \"Rad Gene\" at Kibo-report 3"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム"},{"bibliographic_title":"Space Utilization Research: Proceedings of Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_14":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第26回宇宙利用シンポジウム(2010年1月25日-26日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス)","subitem_description_type":"Other"}]},"item_5_description_15":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）（英）","attribute_value_mlt":[{"subitem_description":"The Twenty-sixth Space Utilization Symposium (January 25-26, 2010: ISAS/JAXA Sagamihara, Japan)","subitem_description_type":"Other"}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"To identify DNA damage induced by space radiations such as the high linear energy transfer (LET) particles, phospho-H2AX (gammaH2AX) foci formation was analyzed in human cells frozen in an International Space Station freezer for 133 days. After recovering the frozen sample to the earth, the cells were cultured for 30 min, and then fixed. Here, we show a track of gammaH2AX positive foci in them by immuno-cytochemical methods. It is suggested that space radiations, especially high LET particles, induced DSBs as a track. From the formation of the tracks in nuclei, exposure dose rate was calculated to be 0.7 mSv per day as relatively high-energy space radiations of Fe-ions (500 MeV/u, 200 keV/μm). From the physical dosimetry with CR-39 plastic nuclear track detectors and thermo-luminescent dosimeters, dose rate was 0.5 mSv per day. These values were similar between biological and physical dosimetries. In addition, the aim of this study was to clarify the effect of space radiations on the radio-adaptive response. Two human lymphoblastoid cell lines were used; one cell line bears a wild-type p53 (wtp53) gene, and another cell line bears a mutated p53 (mp53) gene. The frozen cells were kept in spaceflight on Space Shuttle and International Space Station (ISS) freezer for 133 days from Nov 15th, 2008 to Mar 29th, 2009. After the frozen samples were returned to earth, the cells were cultured for 6 h, and then exposed to challenging X-irradiation doses of 1.2 Gy or 2 Gy. Cellular sensitivity, apoptosis and chromosome aberrations were scored using a dye-exclusion assay, Hoechst33342 staining assay, and chromosomal banding techniques, respectively. In the cells exposed to a space environment, all of radio-adaptive responses such as the induction of radio-resistance and the depression of radiation-induced apoptosis, chromosome aberrations and mutant frequencies investigated here were found in wtp53 cells, but not in the mp53 cells. These results confirmed that the cells exposed to a space environment were likely to the exposed cells to radiation in the specific low dose range which can lead to an adaptive response on ground-base experiments, and that the cells preserved an effect from the space-radiation exposure, even at such low doses in space.","subitem_description_type":"Other"}]},"item_5_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"},{"subitem_description":"共催: 日本学術会議","subitem_description_type":"Other"},{"subitem_description":"著者人数: 12人","subitem_description_type":"Other"}]},"item_5_description_19":{"attribute_name":"内容記述（英）","attribute_value_mlt":[{"subitem_description":"Physical characteristics: Original contains color illustrations","subitem_description_type":"Other"},{"subitem_description":"joint hosting: The Science Council of Japan","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0064730096","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)"}]},"item_5_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10324210","subitem_source_identifier_type":"NCID"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"奈良県立医科大学 : 宇宙航空研究開発機構"},{"subitem_text_value":"奈良県立医科大学 : 宇宙航空研究開発機構"},{"subitem_text_value":"奈良県立医科大学"},{"subitem_text_value":"放射線医学総合研究所"},{"subitem_text_value":"放射線医学総合研究所"},{"subitem_text_value":"日本宇宙フォーラム宇宙利用事業部生命科学研究グループ : 鹿児島大学"},{"subitem_text_value":"日本宇宙フォーラム"},{"subitem_text_value":"鹿児島大学 : エイ・イー・エス"},{"subitem_text_value":"鹿児島大学 : エイ・イー・エス"},{"subitem_text_value":"宇宙航空研究開発機構有人宇宙環境利用ミッション本部宇宙医学生物学研究室"},{"subitem_text_value":"宇宙航空研究開発機構宇宙科学研究本部ISS科学プロジェクト室"},{"subitem_text_value":"宇宙航空研究開発機構宇宙科学研究本部ISS科学プロジェクト室 : 鹿児島大学"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nara Medical University : Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Nara Medical University : Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Nara Medical University"},{"subitem_text_language":"en","subitem_text_value":"National Institute of Radiological Sciences"},{"subitem_text_language":"en","subitem_text_value":"National Institute of Radiological Sciences"},{"subitem_text_language":"en","subitem_text_value":"Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum :  Kagoshima University"},{"subitem_text_language":"en","subitem_text_value":"Life Science Research Group, Space Utilization Promotion Department,Japan Space Forum"},{"subitem_text_language":"en","subitem_text_value":"Kagoshima University : Advanced Engineering Services (AES)"},{"subitem_text_language":"en","subitem_text_value":"Kagoshima University : Advanced Engineering Services (AES)"},{"subitem_text_language":"en","subitem_text_value":"JAXA Space Biomedical Research Office, Human Space Systems and Utilization Mission Directorate, Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"ISS Science Project Office, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)"},{"subitem_text_language":"en","subitem_text_value":"ISS Science Project Office, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS) : Kagoshima University"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大西, 武雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高橋, 昭久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 雅雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鶴岡, 千鶴"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, ひろみ"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"嶋津, 徹"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"関, 真也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"橋爪, 藤子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"永松, 愛子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大森, 克徳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石岡, 憲昭"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onishi, Takeo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Akihisa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Su, Xiaoming","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuruoka, Chizuru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Hiromi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimazu, Toru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seki, Masaya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashizume, Toko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagamatsu, Aiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omori, Katsunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishioka, Noriaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"64730096.pdf","filesize":[{"value":"765.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"64730096.pdf","url":"https://jaxa.repo.nii.ac.jp/record/13243/files/64730096.pdf"},"version_id":"27a1d453-e0bb-40d7-a659-4ab3ec44ea2a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"International Space Station, space radiations, DNA damage, radio-adaptive response, p53","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"「きぼう」でのRad Gene 宇宙実験報告3","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"「きぼう」でのRad Gene 宇宙実験報告3"}]},"item_type_id":"5","owner":"1","path":["1641","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13243","relation_version_is_last":true,"title":["「きぼう」でのRad Gene 宇宙実験報告3"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T06:01:47.799250+00:00"}