@inproceedings{oai:jaxa.repo.nii.ac.jp:00013252, author = {藤井, 伸治 and 渡辺, 千秋 and 矢内, 健一 and 宮沢, 豊 and 高橋, 秀幸 and Fujii, Nobuharu and Watanabe, Chiaki and Yanai, Kenichi and Miyazawa, Yutaka and Takahashi, Hideyuki}, book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium}, month = {Mar}, note = {第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan), When cucumber seeds are placed in a horizontal position for germination, resulting seedlings develop a specialized protuberance, termed the peg, on the lower side of the transition zone between the hypocotyl and the root, due to gravistimulation. On the other hand, in our space-flight experiment of STS-95, cucumber seedlings grown under microgravity conditions developed a peg on each side of the transition zone, suggesting that gravistimulation was required for suppressing the peg formation on the upper side of the transition zone. We have shown that auxin induces peg formation. In addition, the decrease in mRNA accumulation of auxin-inducible CsIAA1 gene was detected in the upper side of the transition zone of horizontally-grown cucumber seedlings, whereas the decrease in CsIAA1 transcript level was not found in the transition zone of space-grown seedlings. We have therefore proposed that gravistimulation induces the decrement of IAA in the upper side of the transition zone and thus suppresses peg formation. However, the auxin distribution in the transition zone during peg formation is still unknown. To reveal the IAA distribution upon gravistimulation in the transition zone, we are now investigating the auxin distribution by immunofluorescence method using anti-IAA monoclonal antibody and will present our recent results about that., 資料番号: AA0064297008}, publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)}, title = {キュウリの芽ばえの重力形態形成にともなうオーキシン分布の重力応答性の抗オーキシン抗体を用いた免疫組織的解析}, volume = {25}, year = {2009} }