{"created":"2023-06-20T14:45:46.312579+00:00","id":13259,"links":{},"metadata":{"_buckets":{"deposit":"1c260166-b06f-4c2b-ae68-46a7b0ab206b"},"_deposit":{"created_by":1,"id":"13259","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13259"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013259","sets":["1543:1633:1642","1887:1891"]},"author_link":["120039","120043","120045","120038","120040","120037","120036","120044","120042","120041"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Sensitive detection ofDNA double-strand breakage and its repair"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム"},{"bibliographic_title":"Space Utilization Research: Proceedings of Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_14":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス)","subitem_description_type":"Other"}]},"item_5_description_15":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）（英）","attribute_value_mlt":[{"subitem_description":"The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan)","subitem_description_type":"Other"}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"It is necessary to develop a sensitive detection method of biological damage of cosmic radiation. Among radiation damages, DNA double-strand breakage is the most important because it leads to cell death, mutagenesis and carcinogenesis. We have been developing a very sensitive method for detection of DNA double-strand breakage. A bacterial (Escherichia coli) genome is made of a circular double-stranded DNA of 4,000,000 bp. This huge circle is easily trapped in the trees of agarose resin and cannot move in a electric field. However, a single double-strand break would make it a linear form, which can move through agarose under an alternating electric field. This represents an application of pulsed-field gel electrophoresis. Our goal is to establish this method for sensitive detection of DNA double-strand breakage in vivo. (1) We detected conditions for detection of chromosomal DNA double-strand breakage by pulsed-field gel electrophoresis. (2) We measured 'background' DNA double-strand breakage under a standard condition of bacterial growth. We used densitometry. We confirmed accumulation of broken chromosomes in a recBC mutant. (3) We measured DNA double-strand breakage after its artificial induction by methyl-specific endonuclease and UV irradiation. These results indicate that this represents a very sensitive method for detection of chromosomal double-stranded DNA breakage. We can try experiments in space to evaluate potential drawbacks of this method. In such cases, we need to find out further details, that includes appropriate physiological conditions for the bacterial cells and their containers. In order to increase sensitivity of this method, it is necessary to reduce the background chromosome breakage. We need to understand biology of chromosomal double-strand breakage, including programmed death in bacteria and damage repair.","subitem_description_type":"Other"}]},"item_5_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0064297015","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)"}]},"item_5_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10324210","subitem_source_identifier_type":"NCID"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院新領域創成科学研究科メディカルゲノム専攻"},{"subitem_text_value":"東京大学大学院新領域創成科学研究科メディカルゲノム専攻"},{"subitem_text_value":"東京大学大学院新領域創成科学研究科メディカルゲノム専攻"},{"subitem_text_value":"東京大学大学院新領域創成科学研究科メディカルゲノム専攻"},{"subitem_text_value":"東京大学大学院新領域創成科学研究科メディカルゲノム専攻"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Science, University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Science, University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Science, University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Science, University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Science, University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 一三"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"半田, 直史"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"河合, 幹彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高橋, 規子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"福田, 江里"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Ichizo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Handa, Naofumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawai, Mikihiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda, Eri","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"64297015.pdf","filesize":[{"value":"451.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"64297015.pdf","url":"https://jaxa.repo.nii.ac.jp/record/13259/files/64297015.pdf"},"version_id":"69d57618-7935-452e-ad9d-a674e2e9133d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA repair, bacteria, DNA damage","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"ゲノムDNA二重鎖切断とその修復の高感度検出法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ゲノムDNA二重鎖切断とその修復の高感度検出法の開発"}]},"item_type_id":"5","owner":"1","path":["1642","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13259","relation_version_is_last":true,"title":["ゲノムDNA二重鎖切断とその修復の高感度検出法の開発"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T06:01:15.537953+00:00"}