@inproceedings{oai:jaxa.repo.nii.ac.jp:00013275,
 author = {山口, 進康 and 一條, 知昭 and 永瀬, 裕康 and 馬場, 貴志 and 那須, 正夫 and Yamaguchi, Nobuyasu and Ichijo, Tomoaki and Nagase, Hiroyasu and Baba, Takashi and Nasu, Masao},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Mar},
 note = {第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan), A Controlled Ecological Life Support System (CELSS) is essential for habitation in extreme environments (space, polar regions, ocean depths, etc.) and an enormous amount of freshwater is used and regenerated for drinking and living. In order to assure human health in closed habitations, microbiological quality control of freshwater used in CELSS is required, and bacterial population dynamics in the system should be determined rapidly and accurately. In this study, bacterial abundance and the numbers of bacteria with physiological activity in freshwater used and regenerated in Closed Ecology Experiment Facilities (CEEF; Aomori, Japan) during the Closed Habitation Experiments were determined to understand bacterial abundance and activity in a closed habitation. Our results suggest that bacterial numbers and their activities in freshwater used in CELSS could be accurately determined within a few hours by fluorescent vital staining. PCR-DGGE (denaturing gradient gel electrophoresis) analyses revealed that alpha-proteobacteria was abundant in freshwater and hydroponics used in the CEEF. Rapid microbiological methods would contribute to the technical progress in the quality assurance of freshwater used in CELSS. For further simple and automated microbiological monitoring, we developed a microfluidic system for quantification of bacteria in freshwater. Comparisons of counts of Escherichia coli by the microfluidic system and by fluorescence microscopy closely correlated (r(exp 2) = 0.98). Bacteria in purified household tap water were rapidly and accurately counted by using this system with on-chip fluorescent staining. A culture-independent DNA fingerprinting technique, Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis is widely used for profiling bacterial populations in aquatic environments. Capillary electrophoresis systems are usually used for the T-RFLP analysis; however this system is rather time-consuming. We developed a protocol for T-RFLP analysis using a microchip electrophoresis system. The time required for this analysis was 15 min, while 80 min was required for conventional T-RFLP analysis using a capillary electrophoresis system. These methods might contribute to technical progress in the microbiological quality control of freshwater used in closed habitations., 形態: カラー図版あり, 資料番号: AA0064297031},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {閉鎖生態系生命維持システム(CELSS)における水の衛生微生物学的安全性評価システムの開発},
 volume = {25},
 year = {2009}
}