{"created":"2023-06-20T14:45:47.552016+00:00","id":13287,"links":{},"metadata":{"_buckets":{"deposit":"4366a824-7491-40e2-a8d4-5491f0570282"},"_deposit":{"created_by":1,"id":"13287","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13287"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013287","sets":["1543:1633:1642","1887:1891"]},"author_link":["120394","120397","120395","120393","120396","120398"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Dynamics of molecular chaperone alpha B-crystallin, which maintains the anti-gravity muscle on the ground, and the change in the space"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム"},{"bibliographic_title":"Space Utilization Research: Proceedings of Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_14":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス)","subitem_description_type":"Other"}]},"item_5_description_15":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）（英）","attribute_value_mlt":[{"subitem_description":"The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan)","subitem_description_type":"Other"}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Disuse-muscle atrophy, especially both in space and in aged peoples on the 1 G earth, is severe problems not only to keep cell survival in our body working in activity-dependent fashion, but also to maintain whole body activities. Alpha B-crystallin, one of small heat shock proteins, is constitutively expressed in heart and slow skeletal muscles, both express slow myosin with high oxidative metabolism, resulting in creating high endurance, and functions as molecular chaperone necessary for endurance. During stress, such as heat, oxidative, and mechanical stresses, alpha B-crystallin protects proteins from stress-induced denature and aggregate, in consequence, improves the cell survival. It has been reported that alpha B-crystallin is involved in the stabilization and the regulation of cytoskeleton, such as intermediate filament, actin and tubulin/microtubule, especially their free forms. Two cytoskeleton systems of actin and tubulin/MT, which are called as \"treadmilling\" or \"dynamic instability\", are maintained dynamic condition with chemical equilibrium of free and polymerized forms, at least in proliferating cells. Recently it has been reported that alpha B-crystallin displays a very distinct cross-striated sarcomeric staining after severe heat shock (45 C). Since alpha B-crystallin does not associate with free tubulin of soleus muscle cells under 4 C but under 37 C by immunoprecipitation analysis shown in our previous work. From these results, conditions of inner environment in our mammalian body are workable in narrow conditions to produce homeostasis, which is kept with molecular chaperone essential for protein homeostasis. The purpose of the present study is to investigate the dynamic view of alpha B-crystallin in living beating myocardial cells under both normal temperature and heat shock conditions by the use of time-lapse imaging system. We used green fluorescent protein (GFP) fused with alpha B-crystallin and it was expressed in rat neonatal myocardial cells. GFP-alpha B-crystallin transiently expressed in beating cardiac myocyte showed a striated pattern under normal condition. In contrast, GFP alone did not show a striated pattern in the same condition. Moreover, to investigate dynamic property of alpha B-crystallin in cardiac myocytes, fluorescent recovery after photobleaching (FRAP) analysis and fluorescent loss in photobleaching (FLIP) analysis of GFP-alpha B-crystallin indicated that the mobility of GFP-alpha B-crystallin in normal state is faster than during heat shock. These results demonstrated that the function of alpha B-crystallin is to work as molecular chaperone for structural proteins constituting of Z-bands of sarcomere and/or cytoskeleton, which should be also dynamically regulated during normal continuous beating. Beating itself under body temperature may be associated with folding/unfolding of Z-band proteins including the cytoskeleton. Both antigravity soleus and heart muscles highly express three proteins of tubulin, alpha B-crystallin and slow (alpha/beta myosin). One of factors to induce atrophy in space will be related to convection, which may influence the changes of protein dynamic, typically shown in cytoskeleton systems. Changes of sHSPs including alpha B-crystallin may good indicator to assume subtle and difficult mechanism to understand gravity-relating mechanism of our dynamic animal organism in space.","subitem_description_type":"Other"}]},"item_5_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: カラー図版あり","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0064297043","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)"}]},"item_5_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10324210","subitem_source_identifier_type":"NCID"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院情報理工学系研究科"},{"subitem_text_value":"東京大学大学院総合文化研究科"},{"subitem_text_value":"東京大学サステイナビリティ学連携研究機構"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Graduate School of Information Science and Technology, University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Arts and Sciences, University of Tokyo"},{"subitem_text_language":"en","subitem_text_value":"Department of Integrated Research System for Sustainability Science (IR3S), University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤田, 恵理"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"桜井, 隆史"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"跡見, 順子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujita, Eri","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Atomi, Yoriko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"64297043.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"64297043.pdf","url":"https://jaxa.repo.nii.ac.jp/record/13287/files/64297043.pdf"},"version_id":"3a80e709-5843-42ee-b630-6877fc927515"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"地球環境下で抗重力筋を維持する分子シャペロンαB-crystallinのダイナミクスと宇宙での変化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"地球環境下で抗重力筋を維持する分子シャペロンαB-crystallinのダイナミクスと宇宙での変化"}]},"item_type_id":"5","owner":"1","path":["1642","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13287","relation_version_is_last":true,"title":["地球環境下で抗重力筋を維持する分子シャペロンαB-crystallinのダイナミクスと宇宙での変化"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T06:00:20.071973+00:00"}