@inproceedings{oai:jaxa.repo.nii.ac.jp:00013306,
 author = {清本, 正人 and 黒谷, 明美 and 江口, 星雄 and 山口, 守 and Kiyomoto, Masato and Izumi-Kurotani, Akemi and Eguchi, Hoshio and Yamaguchi, Mamoru},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Mar},
 note = {第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan), Sea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicules grow up to larval skeletons. The skeletogenic cells are called primary mesenchyme cells derived from micromeres at 16-cell stage. We already reported a promotive effect of hypergravity on skeletogenesis in the 0.25 % horse serum culture of the skeletogenic cells. In this condition, cultured skeletogenic cells are so sensitive that they show a clear difference of skeletogenesis under the hypergravity though it is difficult to detect significant difference in the culture medium containing 4 % horse serum. In 0.25 % serum, the number of spicule is less than that in 4 % serum under 1 xg condition. It recovers to the similar level significantly even in 0.25 % under hypergravity(100 xg). In this study, we examined the method for detecting the effect of hypergravity on the skeleton formation of the sea urchin juvenile. The skeletons of 8 armed-larvae with full-grown echinus rudiment were stained by calcein. Then, they were cultured under hypergravity with coralline red algae for inducing metamorphosis. The growth of skeleton during under hypergravity was measured at the tip of spines, where no calcein staining is observed. The growth of spine is from 40 to 100 micrometers depending on the size of test of each juvenile., 形態: カラー図版あり, 資料番号: AA0064297062},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {ウニの成体骨格に対する重力環境の影響の検討},
 volume = {25},
 year = {2009}
}