@inproceedings{oai:jaxa.repo.nii.ac.jp:00013307,
 author = {鈴木, 信雄 and 田畑, 純 and 大森, 克徳 and 井尻, 憲一 and 北村, 敬一郎 and 根本, 鉄 and 清水, 宣明 and 染井, 正徳 and 池亀, 美華 and 中村, 正久 and Suzuki, Nobuo and Tabata, Makoto J. and Omori, Katsunori and Ijiri, Kenichi and Kitamura, Keiichiro and Nemoto, Tetsu and Shimizu, Nobuaki and Somei, Masanori and Ikegame, Mika and Nakamura, Masahisa},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Mar},
 note = {第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan), Fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammalian membrane bone. Recently, we developed a new in vitro model system using goldfish scales. This system can detect the activities of osteoblasts and osteoclasts with alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective markers and can analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we previously indicated that osteoblastic and osteoclastic activities in the scales responded to simulated microgravity with a three-dimensional clinostat. As these activities in the scales were not changed by stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in scale osteoblasts and osteoclasts is a specific phenomenon. Thus, to analyze bone metabolism in scales under microgravity conditions such as those experienced during space flights, we examined culture conditions in goldfish scales. We found that normal scales and regenerating scales treated by hypochlorous acid could be cultured in Leibovitz L-15 medium supplemented with 10 % fetal bovine serum for 1 week at 25 C. After these scales were kept in the above medium at 4 C, the response of hyper-gravity loading under acceleration by vibration (3 G, 10 min) was examined. The TRAP activities in the normal and regenerating scales kept at 4 C for 1 week significantly decreased, while ALP activities in these scales significantly increased. These responses in the scales kept at 4 C for 1 week were very similar to those in the untreated scales just after collection from goldfish. We concluded that fish scales are a suitable material for the analysis of bone metabolism under microgravity conditions, such as those experienced during space flights., 著者人数: 25人, 資料番号: AA0064297063},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {魚類のウロコを用いた宇宙生物学的研究: 宇宙実験に適したウロコの培養法の検討},
 volume = {25},
 year = {2009}
}