@inproceedings{oai:jaxa.repo.nii.ac.jp:00013319, author = {塚本, 勝男 and 佐崎, 元 and 小島, 謙一 and 橘, 勝 and 吉崎, 泉 and Tsukamoto, Katsuo and Sazaki, Gen and Kojima, Kenichi and Tachibana, Masaru and Yoshizaki, Izumi}, book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium}, month = {Mar}, note = {第25回宇宙利用シンポジウム(2009年1月14日-15日, 宇宙航空研究開発機構宇宙科学研究本部相模原キャンパス), The Twenty-fifth Space Utilization Symposium (January 14-15, 2009: ISAS/JAXA Sagamihara, Japan), Growth rate of lysozyme crystals has been measured for the first time under microgravity by growing seed crystals. Unexpectedly growth rate under microgravity was the same as that under gravity or even larger under gravity. Precise growth rate of lysozyme crystals under microgravity has successfully been measured for the first time using a Russian Foton-M3 satellite in September 2007. The growth rate was obtained by measuring the thickness increase of seed crystals during the microgravity duration, 12 days, after the transportation of growth cells from ESTEC, The Netherlands, to Baikonur, Russia. All growth cells were kept at 20 C after preparation of the seed crystals. The growth patterns at various stage of microgravity and gravity experiments in the same crystals overgrown on the seeds were also been observed for the first time by laser confocal microscopy after the flight. The shape of growth step patterns was used to analyze the effect of impurity on the growth of crystals. Some of space grown crystals were examined also by synchrotron X-ray topography at KEK in Tsukuba, after all optical observations were completed. This was needed to analyze the origin of defects in crystals in relation to the growth mechanism of the crystals. More than 50 seed crystals have been selected for the microgravity experiments. These seeds in various concentrations of lysozyme (98.5 % purity) were sealed in glass tubes, phi = 3 mm, which enabled us to grow the seed crystals at constant temperature, 20 C and thus at known initial supersaturation. After seeding on the ground, the seed crystals continued to grow under 1 G and then under micro G after launch. Because of the change of growth conditions during the experiment, the refractive index of the crystals slightly changed and thus growth striations are visible, fig.1, if the growth interface is viewed from the side of the crystal parallel to the optical axis of the confocal microscopy. It was surprising to see that the growth rates under both gravity levels were equal or even larger under micro G than under 1 G. This was interpreted based on the coupling effects of largely reduced impurity concentrations at the surface and the less reduced growth rate of crystals in space., 形態: カラー図版あり, 資料番号: AA0064297075}, publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)}, title = {フォトンM3衛星を使ったタンパク質結晶成長速度測定}, volume = {25}, year = {2009} }