@inproceedings{oai:jaxa.repo.nii.ac.jp:00013435,
 author = {鈴木, 信雄 and 大森, 克徳 and 井尻, 憲一 and 北村, 敬一郎 and 清水, 宣明 and 田畑, 純 and 池亀, 美華 and 中村, 正久 and 近藤, 隆 and 松田, 恒平 and Suzuki, Nobuo and Omori, Katsunori and Ijiri, Kenichi and Kitamura, Keiichiro and Shimizu, Nobuaki and Tabata, Makoto J. and Ikegame, Mika and Nakamura, Masahisa and Kondo, Takashi and Matsuda, Kohei},
 book = {宇宙利用シンポジウム　第24回　平成19年度, Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium},
 month = {Mar},
 note = {Fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammalian bone. Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoblasts and osteoclasts with AlkaLine Phosphatase (ALP) and Tartrate-Resistant Acid Phosphatase (TRAP) as the respective markers and analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we indicated that osteoblastic and osteoclastic activities in the scale responded to the simulated microgravity with a three-dimensional clinostat. As these activities in the scale did not change by the stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in the scale osteoblasts and osteoclasts is a specific phenomenon. In addition, we analyzed the bone metabolism under 2-, 4-, and 7-gravity (G) loading with a centrifuge and compared them with the control (1-G). The osteoblastic activity significantly increased under 2- and 4-G loading. This activity increased remarkably under 7-G loading. On the other hand, we found that the osteoclastic activity significantly decreased under 2-G loading. Under 4-G loading, there was no significant difference between G-loaded scales and control scales. The osteoclastic activity tended to increase under 7-G loading. These results were similar to our previous study of acceleration by vibration. With the goal of developing a drug for bone diseases, the effect of novel bromomelatonin derivatives on osteoblasts and osteoclasts was examined. All bromomelatonin derivatives had an inhibitory action on osteoclasts. In particular, 1-benzyl-2, 4, 6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action. In addition, estrogen receptor mRNA (messenger Ribonucleic Acid) expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(exp -7) M)-treated scales. Therefore, this chemical will be useful to treat bone diseases, such as those experienced in space flight., 資料番号: AA0063706059},
 pages = {230--233},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)},
 title = {擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析},
 year = {2008}
}