{"created":"2023-06-20T14:45:56.229154+00:00","id":13435,"links":{},"metadata":{"_buckets":{"deposit":"546bc50c-e577-432d-a624-1c464692792e"},"_deposit":{"created_by":1,"id":"13435","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13435"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013435","sets":["1543:1633:1643","1887:1891"]},"author_link":["122412","122424","122415","122420","122418","122413","122422","122411","122416","122423","122425","122407","122421","122406","122419","122414","122410","122409","122417","122408"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Regulation of bone metabolism under simulated micro-gravity and hyper-gravity loading: Analysis by the cultured scales of goldfish"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"233","bibliographicPageStart":"230","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第24回　平成19年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammalian bone. Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoblasts and osteoclasts with AlkaLine Phosphatase (ALP) and Tartrate-Resistant Acid Phosphatase (TRAP) as the respective markers and analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we indicated that osteoblastic and osteoclastic activities in the scale responded to the simulated microgravity with a three-dimensional clinostat. As these activities in the scale did not change by the stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in the scale osteoblasts and osteoclasts is a specific phenomenon. In addition, we analyzed the bone metabolism under 2-, 4-, and 7-gravity (G) loading with a centrifuge and compared them with the control (1-G). The osteoblastic activity significantly increased under 2- and 4-G loading. This activity increased remarkably under 7-G loading. On the other hand, we found that the osteoclastic activity significantly decreased under 2-G loading. Under 4-G loading, there was no significant difference between G-loaded scales and control scales. The osteoclastic activity tended to increase under 7-G loading. These results were similar to our previous study of acceleration by vibration. With the goal of developing a drug for bone diseases, the effect of novel bromomelatonin derivatives on osteoblasts and osteoclasts was examined. All bromomelatonin derivatives had an inhibitory action on osteoclasts. In particular, 1-benzyl-2, 4, 6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action. In addition, estrogen receptor mRNA (messenger Ribonucleic Acid) expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(exp -7) M)-treated scales. Therefore, this chemical will be useful to treat bone diseases, such as those experienced in space flight.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0063706059","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"金沢大学 自然計測応用研究センター"},{"subitem_text_value":"宇宙航空研究開発機構"},{"subitem_text_value":"東京大学 アイソトープ総合センター"},{"subitem_text_value":"金沢大学 大学院医学系研究科"},{"subitem_text_value":"金沢大学 自然計測応用研究センター"},{"subitem_text_value":"東京医科歯科大学 大学院"},{"subitem_text_value":"岡山大学 大学院医歯薬学総合研究科"},{"subitem_text_value":"早稲田大学 教育・総合科学学術院"},{"subitem_text_value":"富山大学 医学薬学研究部"},{"subitem_text_value":"富山大学 大学院理工学研究科"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Kanazawa University Institute of Nature and Environmental Technology"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency"},{"subitem_text_language":"en","subitem_text_value":"University of Tokyo Radioisotope Center"},{"subitem_text_language":"en","subitem_text_value":"Kanazawa University Graduate School of Medical Science"},{"subitem_text_language":"en","subitem_text_value":"Kanazawa University Institute of Nature and Environmental Technology"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University Graduate School"},{"subitem_text_language":"en","subitem_text_value":"Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences"},{"subitem_text_language":"en","subitem_text_value":"Waseda University Faculty of Education and Integral Arts and Science"},{"subitem_text_language":"en","subitem_text_value":"University of Toyama Graduate School of Medicine and Pharmaceutical Sciences"},{"subitem_text_language":"en","subitem_text_value":"University of Toyama Graduate School of Science and Engineering"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 信雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大森, 克徳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"井尻, 憲一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"北村, 敬一郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"清水, 宣明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田畑, 純"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"池亀, 美華"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中村, 正久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"近藤, 隆"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松田, 恒平"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omori, Katsunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ijiri, Kenichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitamura, Keiichiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimizu, Nobuaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tabata, Makoto J.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikegame, Mika","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Masahisa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuda, Kohei","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"63706059.pdf","filesize":[{"value":"379.5 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scale","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fish","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metabolism","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell culturing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"clinostat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"centrifuge","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high gravity environment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bromomelatonin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carassius auratus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gravitational effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"activity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference 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