{"created":"2023-06-20T14:45:58.338343+00:00","id":13474,"links":{},"metadata":{"_buckets":{"deposit":"a7cd1558-c0fb-4294-aa9f-99e946cf6d81"},"_deposit":{"created_by":1,"id":"13474","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13474"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013474","sets":["1543:1633:1643","1887:1891"]},"author_link":["122907","122905","122909","122906","122902","122908","122904","122903"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Rapid and accurate monitoring of bacteria"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"350","bibliographicPageStart":"347","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第24回　平成19年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"A Controlled Ecological Life Support System (CELSS) is essential for habitation in extreme environments (space, polar regions, ocean depths, etc.) and an enormous amount of freshwater is used and regenerated for drinking and living. In order to assure human health, microbiological quality control of freshwater used in CELSS is required, and bacterial population dynamics in the system should be determined rapidly and accurately. In this study, bacterial abundance and the numbers of bacteria with physiological activity in freshwater used and regenerated in Closed Ecology Experiment Facilities (CEEF; Aomori, Japan) during the Closed Habitation Experiments were determined to understand bacterial abundance and activity in a closed habitation. The numbers of total bacteria and esterase-active bacteria in each sample were determined by fluorescent staining techniques. Colony-forming units were determined by the plate-counting technique and growing cells were also counted by the microcolony method. Our results suggest that bacterial numbers and their activities in freshwater used in CELSS could be accurately determined within a few hours by fluorescent vital staining. Rapid microbiological methods would contribute to the technical progress in the quality assurance of freshwater used in CELSS. For further simple and automated microbiological monitoring, we developed a simplified microfluidic device for quantification of bacteria in potable water. Comparisons of counts of Escherichia coli by the microfluidic system and by fluorescence microscopy closely correlated (r (exp 2) = 0.99). Bacteria in natural mineral water and in purified household tap water were accurately enumerated by using this system within 15 min after fluorescent staining.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0063706098","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"大阪大学 大学院薬学研究科"},{"subitem_text_value":"大阪大学 大学院薬学研究科"},{"subitem_text_value":"大阪大学 大学院薬学研究科"},{"subitem_text_value":"環境科学技術研究所"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Osaka University Graduate School of Pharmaceutical Sciences"},{"subitem_text_language":"en","subitem_text_value":"Osaka University Graduate School of Pharmaceutical Sciences"},{"subitem_text_language":"en","subitem_text_value":"Osaka University Graduate School of Pharmaceutical Sciences"},{"subitem_text_language":"en","subitem_text_value":"Institute for Environmental Sciences"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山口, 進康"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"馬場, 貴志"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"那須, 正夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"多胡, 靖宏"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Nobuyasu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Baba, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nasu, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tako, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-20"}],"displaytype":"detail","filename":"63706098.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"63706098.pdf","url":"https://jaxa.repo.nii.ac.jp/record/13474/files/63706098.pdf"},"version_id":"d8b6a594-38a8-493e-a4ab-ddc0193b8a20"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"コロニー","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞培養","subitem_subject_scheme":"Other"},{"subitem_subject":"染色","subitem_subject_scheme":"Other"},{"subitem_subject":"計数","subitem_subject_scheme":"Other"},{"subitem_subject":"細菌学","subitem_subject_scheme":"Other"},{"subitem_subject":"環境モニタリング","subitem_subject_scheme":"Other"},{"subitem_subject":"微生物","subitem_subject_scheme":"Other"},{"subitem_subject":"閉鎖生態系","subitem_subject_scheme":"Other"},{"subitem_subject":"精度","subitem_subject_scheme":"Other"},{"subitem_subject":"微生物モニタリング","subitem_subject_scheme":"Other"},{"subitem_subject":"コロニー形成細菌数","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロ流路システム","subitem_subject_scheme":"Other"},{"subitem_subject":"細菌数","subitem_subject_scheme":"Other"},{"subitem_subject":"bacterium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"colony","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell culturing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"staining","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"counting","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bacteriology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"environmental monitoring","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microorganism","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"closed ecological system","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"accuracy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microbiological monitoring","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"colony forming unit","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microfluidic system","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bacterial number","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"迅速・高精度な細菌モニタリング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"迅速・高精度な細菌モニタリング"}]},"item_type_id":"5","owner":"1","path":["1643","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13474","relation_version_is_last":true,"title":["迅速・高精度な細菌モニタリング"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T05:54:12.575156+00:00"}