@inproceedings{oai:jaxa.repo.nii.ac.jp:00013585,
 author = {谷田貝, 文夫 and 梅林, 志浩 and 岩木, 正哉 and 本間, 正充 and 鈴木, 雅雄 and 石岡, 憲昭 and 嶋津, 徹 and 鈴木, ひろみ and Yatagai, Fumio and Umebayashi, Yukihiro and Iwaki, Masaya and Honma, Masamitsu and Suzuki, Masao and Ishioka, Noriaki and Shimazu, Toru and Suzuki, Hiromi},
 book = {宇宙利用シンポジウム　第23回　平成18年度, Space Utilization Research: Proceedings of the Twenty-third Space Utilization Symposium},
 month = {Mar},
 note = {DSB (Double Strand Break) was introduced by the I-Sce I expression vector, pCMV3xnls-I-Sce I. Repair of DSB at the I-SceI site inserted at intron 4 of the tk+ allele in human lymphoblstoid TK6 cell line, TSCE5, was measured by induction of TK-deficient mutants. Similarly we also measured the revertants due to such repair in its compound heterozygote (tk-/-) cell line, TSCER2, which carried an additional point-mutation in exon 5. The former and later measurements reflect the DSB repair efficiency due to DNA End-Joining (EJ) and Homologous Recombination (HR), respectively. The pre-treatment, gamma-irradiation with 30 mGy at a dose rate of 1.2 mGy/hr did not influence the EJ repair efficiency but enhanced HR repair efficiency, approximately 50 percent. The I-SceI digestion followed by much lower dose(/dose-rate) gamma-irradiation (8.5 mGy at 0.125 mGy/hr) during the incubation tesulted in a similar tendency of HR enhancement (approximately 50 percent). This kind of treatment, introducing the chromosome break by the restriction enzyme, can be regarded as a useful approach for elucidating the influences of space environments on the DSB repair., 資料番号: AA0063349087},
 pages = {305--308},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)},
 title = {染色体切断の修復は細胞の低線量/低線量率γ線照射による影響を受ける},
 year = {2007}
}