@inproceedings{oai:jaxa.repo.nii.ac.jp:00013593, author = {清本, 正人 and 黒谷, 明美 and 江口, 星雄 and 山口, 守 and Kiyomoto, Masato and IzumiKurotani, Akemi and Eguchi, Hoshio and Yamaguchi, Mamoru}, book = {宇宙利用シンポジウム 第23回 平成18年度, Space Utilization Research: Proceedings of the Twenty-third Space Utilization Symposium}, month = {Mar}, note = {In sea urchin embryo, the skeletogenic cells are called primary meshenchymen cells derived from micromeres at 16-cell stage. We reported a promotive effect of hypergravity in the low serum concentration culture of the skeletogenic cells. In this condition, cultured skeletogenic cells were so sensitive that they showed a clear difference of skeletogenesis also for the different substrate of culture plate. The decrease of spicule number by the inhibitor of Ca(2+)-channel is recovered under hypergravity condition. The culture in low Ca(2+) concentration also decreased the number of formed spicule and the number was recovered under hypergravity, too. These results show the possibility that the Ca(2+) uptake is one of a candidate process that is affected by hypergravity condition. The spicule also contains spicule matrix proteins. The expression of their mRNA under hypergravity was examined and the level was not different from control condition., 資料番号: AA0063349095}, pages = {332--334}, publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)}, title = {過重力環境がウニの骨片形成に与える影響}, year = {2007} }