@inproceedings{oai:jaxa.repo.nii.ac.jp:00013696,
 author = {今井, 真理子 and 黒谷, 明美 and 江口, 星雄 and 山口, 守 and 清本, 正人 and Imai, Mariko and Izumi-Kurotani, Akemi and Eguchi, Hoshio and Yamaguchi, Mamoru and Kiyomoto, Masato},
 book = {宇宙利用シンポジウム, Space Utilization Research: Proceedings of Space Utilization Symposium},
 month = {Mar},
 note = {第22回宇宙利用シンポジウム(2006年1月17日-19日, 日本学術会議6階会議室 六本木、東京), The Twenty-second Space Utilization Symposium (January 17-19, 2006: Science Council of Japan, Roppongi, Tokyo, Japan), Sea urchin and other echinoderm animals have calcitic endoskeleton. In sea urchin embryo, skeletogenesis starts at late gastrula stage and then the spicles grow up to larval skeletons. This skeletogenic cells are called primary mesenchymen cells and are derived from micromeres at 16-cell stage. Spicule formation by the cultured micromere of sea urchin was used as a model for the morphogenesis in biomineralization to be adapted as an experimental system in space. We have detected an effect for spicule formation by cultured micromere in the different gravity environment. In this study we examined the effect of hypergravity in the culture medium with different serum concentration. Though the culture medium containing 4 % horse serum is suitable for the spicule formation, it was difficalt to detect significant difference in hypergravity conditon. In the low concentration of serum the number of spicule decreased and it recovered significantly in hypergravity. It was observed that extra spicules were formed later under hypergravity condition. Next, Ca(2+) transport was examined. The inhibitor of Ca(2+) - channel (diltiazem) was added to the micromere culture. The number of spicules was decreased in the culture with deltiazem. But the decrease of spicule number was recovered under hypergravity condition. These results show the possibility that the Ca(2+) uptake is one of a candidate process that is affected by hypergravity condition., 共催: 日本学術会議, Meeting sponsors: The Science Council of Japan, The Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (ISAS)(JAXA), 資料番号: AA0064113079},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)},
 title = {過重力環境がウニ単離小割球培養細胞やウニの骨片形成に与える影響},
 volume = {22},
 year = {2006}
}