@inproceedings{oai:jaxa.repo.nii.ac.jp:00013773,
 author = {今井, 真理子 and 清本, 正人 and 黒谷, 明美 and 江口, 星雄 and Imai, Mariko and Kiyomoto, Masato and IzumiKurotani, Akemi and Eguchi, Hoshio},
 book = {宇宙利用シンポジウム　第21回　平成16年度, Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium},
 month = {Mar},
 note = {Spicule formation by the cultured micromere of sea urchin was used as a model for the morphogenesis in biomineralization to be adapted as an experimental system in space. Several experiments in the different gravity environment have been carried out to examine a role of gravity in the cells isolated from whole embryo and spicule elongation suppressed in the experiments by centrifugation, clinostat and a series of parabolic flight has been reported. In this study the effect of serum concentration in the culture medium on the sensitivity to hypergravity was examined. Though the culture medium containing 4 percent horse serum is suitable for the spicule formation in the micromere culture, the effect of hypergravity has been shown with various statistical significances. Lower concentration of serum was not enough for full spicule formation by the cultured micromere and the quantity of formed spicule was reduced. But the inhibitory effect of hypergravity was shown more clearly. In this new condition, it seems possible to detect the effect of smaller hypergravity. One of main contents in spicule, calcium, is incorporated from the medium in micromere culture. Higher concentration of calcium recovered spicule formation from the inhibitory effect by the hypergravity. These results show the competitive relation between hypergravity and high calcium concentration., 資料番号: AA0048095044},
 pages = {198--200},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)},
 title = {ウニ胚の単離小割球培養細胞による骨片形成に対する重力環境の影響},
 year = {2005}
}