@inproceedings{oai:jaxa.repo.nii.ac.jp:00013775,
 author = {三浦, ゆり and 加納, まゆみ and 戸田, 年総 and 鈴木, 捷三 and Miura, Yuri and Kano, Mayumi and Toda, Toshifusa and Suzuki, Shozo},
 book = {宇宙利用シンポジウム　第21回　平成16年度, Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium},
 month = {Mar},
 note = {The protein profiles of variously aged rat astrocytes were investigated, which responded to oxidative stress. After hydroperoxide (H2O2)-exposure of cells at 100 micro mols for 30 min, the relative intensity of 10 protein spots changed on 2-D gels compared with control gels after silver staining. MALDI-TOF/MS (Matrix-Assisted Laser Desorption/Ionization - Time Of Flight/Mass Spectrometry) analysis after in-gel digestion revealed that six of these spots corresponded to three kinds of proteins, each of which was composed of a protein and its modified form with a different isoelectric point. These three proteins were identified as Peroxiredoxins (Prxs) 2 and 3, and calpactin 1 light chain (p11). H2O2-exposure increased the intensity of the spot with lower isoelectric point and simultaneously decreased that of the spot with higher isoelectric point for both Prxs 2 and 3. In addition, the expression of annexin 7, S-Adenosyl-L-Homocysteine Hydrolase (SAHH), Elongation Factor 2 (EF-2) fragment, and Adenosine Deaminase (ADA) were increased by H2O2-exposure in glial cells from variously aged rats. Phosphorylated proteins were also analyzed using the Pro-Q Diamond staining, and it was found that Hsp (Heat shock protein) 60 and alpha-tubulin were phosphorylated by H2O2-exposure, and that the phosphorylation of alpha-tubulin was enhanced with aging., 資料番号: AA0048095046},
 pages = {205--208},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)},
 title = {酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析},
 year = {2005}
}