{"created":"2023-06-20T14:46:13.297423+00:00","id":13775,"links":{},"metadata":{"_buckets":{"deposit":"ed2a6ac0-ac5e-4d72-87d2-7dca323d428a"},"_deposit":{"created_by":1,"id":"13775","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13775"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013775","sets":["1543:1633:1646","1887:1891"]},"author_link":["126449","126448","126453","126454","126450","126451","126452","126455"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Proteomics study on the variation of protein expressions and phosphorylations due to oxidative stress"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"208","bibliographicPageStart":"205","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第21回　平成16年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Twenty-first Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The protein profiles of variously aged rat astrocytes were investigated, which responded to oxidative stress. After hydroperoxide (H2O2)-exposure of cells at 100 micro mols for 30 min, the relative intensity of 10 protein spots changed on 2-D gels compared with control gels after silver staining. MALDI-TOF/MS (Matrix-Assisted Laser Desorption/Ionization - Time Of Flight/Mass Spectrometry) analysis after in-gel digestion revealed that six of these spots corresponded to three kinds of proteins, each of which was composed of a protein and its modified form with a different isoelectric point. These three proteins were identified as Peroxiredoxins (Prxs) 2 and 3, and calpactin 1 light chain (p11). H2O2-exposure increased the intensity of the spot with lower isoelectric point and simultaneously decreased that of the spot with higher isoelectric point for both Prxs 2 and 3. In addition, the expression of annexin 7, S-Adenosyl-L-Homocysteine Hydrolase (SAHH), Elongation Factor 2 (EF-2) fragment, and Adenosine Deaminase (ADA) were increased by H2O2-exposure in glial cells from variously aged rats. Phosphorylated proteins were also analyzed using the Pro-Q Diamond staining, and it was found that Hsp (Heat shock protein) 60 and alpha-tubulin were phosphorylated by H2O2-exposure, and that the phosphorylation of alpha-tubulin was enhanced with aging.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0048095046","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京都老人総合研究所"},{"subitem_text_value":"東京都老人総合研究所"},{"subitem_text_value":"東京都老人総合研究所"},{"subitem_text_value":"東京都老人総合研究所"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Tokyo Metropolitan Institute of Gerontology"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Metropolitan Institute of Gerontology"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Metropolitan Institute of Gerontology"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Metropolitan Institute of Gerontology"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三浦, ゆり"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加納, まゆみ"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"戸田, 年総"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 捷三"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Yuri","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kano, Mayumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toda, Toshifusa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Shozo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"酸化ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒドロペルオキシド","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオミクス","subitem_subject_scheme":"Other"},{"subitem_subject":"リン酸化","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオーム","subitem_subject_scheme":"Other"},{"subitem_subject":"電気泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"ペルオキシレドキシン","subitem_subject_scheme":"Other"},{"subitem_subject":"星状細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"ラット","subitem_subject_scheme":"Other"},{"subitem_subject":"oxidative stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hydroperoxide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proteomics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphorylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proteome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"peroxiredoxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"astrocyte","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rat","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析"}]},"item_type_id":"5","owner":"1","path":["1646","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13775","relation_version_is_last":true,"title":["酸化ストレスによるタンパク発現変化とリン酸化の網羅的解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T05:44:59.751225+00:00"}