{"created":"2023-06-20T14:46:15.640163+00:00","id":13812,"links":{},"metadata":{"_buckets":{"deposit":"0db304e7-abe6-4a52-aa51-4cc1bac6cde7"},"_deposit":{"created_by":1,"id":"13812","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13812"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013812","sets":["1543:1633:1647","1887:1891"]},"author_link":["126825","126821","126816","126820","126818","126819","126817","126824","126822","126823"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Pro-and anti-apoptotic response to microgravity in osteoblast cell culture"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"20","bibliographicPageStart":"19","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第20回　平成15年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Twentieth Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The effects of microgravity or simulated microgravity (clinorotation) on the expression of pro- and anti-apoptotic molecules in rat and human osteoblasts were investigated. Rat osteoblasts were cultured aboard space shuttle and solubilized during the mission. The mRNA levels for the inducible nitric oxide synthase (iNOS) and GTP cyclohydrolase 1 (GTPCH) in flight cultures were increased as compared to ground (1 x g) controls. Data of cellular DNA amount suggested that cell death occurred in the earlier days of the mission, however, it was recovered around the days when cells were solubilized on board. Human osteoblasts were cultured under the vector-averaged gravity condition (simulated microgravity) on clinostat rotation. Simulated microgravity increased the pro-apoptotic index (Bax/Bcl-2 ratio) together with the expression of XIAP (inhibitor of apoptosis protein), resulting in no apoptotic DNA fragmentation. In osteoblasts, iNOS is involved in the early response to mechanical strain and induction of apoptosis. GTPCH is essential for iNOS activity, but is coordinately expressed with iNOS to prevent the iNOS-induced apoptosis. Increase of the Bax/Bcl-2 ratio means mitochondrial pro-apoptotic sign. The XIAP binds to the caspase-9 and blocks further apoptotic process. Co-elevation of GTPCH and iNOS, Bax/Bcl-2 and XIAP, may indicate a self-protective features against the pro-apoptotic driving force by microgravity and simulated microgravity.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0046917007","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京医科歯科大学"},{"subitem_text_value":"東京医科歯科大学"},{"subitem_text_value":"東京医科歯科大学"},{"subitem_text_value":"東京医科歯科大学"},{"subitem_text_value":"東京医科歯科大学"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"},{"subitem_text_language":"en","subitem_text_value":"Tokyo Medical and Dental University"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"粂井, 康宏"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中村, 浩"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森田, 定雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大谷, 啓一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"下川, 仁弥太"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kumei, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Sadao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oya, Keiichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimokawa, Hitoyata","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"骨芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"アポトーシス","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞培養","subitem_subject_scheme":"Other"},{"subitem_subject":"クリノスタット","subitem_subject_scheme":"Other"},{"subitem_subject":"ラット","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"スペースシャトル","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell culture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"clinostat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space shuttle","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"微小重力による骨芽細胞のアポトーシス誘導とアポトーシス抑制に関わる分子","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"微小重力による骨芽細胞のアポトーシス誘導とアポトーシス抑制に関わる分子"}]},"item_type_id":"5","owner":"1","path":["1647","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13812","relation_version_is_last":true,"title":["微小重力による骨芽細胞のアポトーシス誘導とアポトーシス抑制に関わる分子"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T05:44:24.943319+00:00"}