{"created":"2023-06-20T14:46:15.837190+00:00","id":13815,"links":{},"metadata":{"_buckets":{"deposit":"08b61f52-4c00-476c-aeb0-58a533c46687"},"_deposit":{"created_by":1,"id":"13815","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13815"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013815","sets":["1543:1633:1647","1887:1891"]},"author_link":["126852","126848","126849","126850","126851","126853"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"The effect of gravity condition for the expression of spicule matrix protein in cultured skeletogenic cells of sea urchin embryo"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"28","bibliographicPageStart":"26","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第20回　平成15年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Twentieth Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Spicule formation by the cultured micromere of sea urchin was used as a model for the morphogenesis in biomineralization to be adapted as an experimental system in space. Several experiments were carried out in the different gravity environment to examine a role of gravity in the cells isolated from whole embryo and reported that spicule elongation was suppressed in the experiments of centrifugation, clinostat and the parabolic flight. The spicules of sea urchin larvae consist of calcite with magnesium and organic substances, called spicule matrix proteins. To examine the detail of the effect of these gravity conditions, RT-PCR was used to measure the level of expression of spicule matrix proteins in the cultured cells under each condition. The expression of one spicule matrix protein (SM30) was suppressed under the centrifugal condition. It also suppressed in the cells whose spicule elongation was suppressed in the culture on the ground after the parabolic flight. These results demonstrate that each gravity condition can effect on the gene expression of spicule matrix protein.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0046917010","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"お茶の水女子大学 理学部附属館山臨界実験所"},{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部"},{"subitem_text_value":"東京大学 原子力研究総合センター"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Ochanomizu University Tateyama Marine Laboratory, Faculty of Science"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency Institute of Space and Astronautical Science"},{"subitem_text_language":"en","subitem_text_value":"University of Tokyo Research Center for Nuclear Science and Technology"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"清本, 正人"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒谷, 明美"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"江口, 星雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiyomoto, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IzumiKurotani, Akemi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Hoshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"重力効果","subitem_subject_scheme":"Other"},{"subitem_subject":"骨片基質細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"胚","subitem_subject_scheme":"Other"},{"subitem_subject":"培養骨格形成細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ウニ","subitem_subject_scheme":"Other"},{"subitem_subject":"遠心分離","subitem_subject_scheme":"Other"},{"subitem_subject":"パラボリックフライト","subitem_subject_scheme":"Other"},{"subitem_subject":"gravitational effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spicule matrix protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"embryo","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured skeletogenic cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sea urchin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"centrifuge","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"parabolic flight","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"ウニ胚の骨片基質タンパク質の発現に対する重力環境の影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウニ胚の骨片基質タンパク質の発現に対する重力環境の影響"}]},"item_type_id":"5","owner":"1","path":["1647","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13815","relation_version_is_last":true,"title":["ウニ胚の骨片基質タンパク質の発現に対する重力環境の影響"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T05:44:21.868967+00:00"}