@inproceedings{oai:jaxa.repo.nii.ac.jp:00013823,
 author = {井上, 明日香 and 秋吉, 英雄 and Inoue, Asuka and Akiyoshi, Hideo},
 book = {宇宙利用シンポジウム　第20回　平成15年度, Space Utilization Research: Proceedings of the Twentieth Space Utilization Symposium},
 month = {Mar},
 note = {Amphibians are widely distributed both geographically and ecologically. Their habitat ranges from water to land, and vertically from apical areas in trees to bottom layers in water. The correlation between the liver structures in the three orders of amphibians and their phylogeny or diverged mode of living was studied by immunohistochemical technique. Methods: Livers of forty-two species amphibians were fixed by perfusion with paraformaldehyde, cut paraffin sections, and observed by light microscope. The cytoskeletal components of the hepatic stellate cells (HSC) and myofibroblasts (MF) were identified by immunohistochemistry for glial fibrillary acidic protein (GFAP) and alpha-smooth muscle actin (alpha-SM actin). The expression of the cytoskeletal components was observed in HSC. Results: In Anura, GFAP-positive HSC were distributed in hepatic lobules and alpha-SM actin-positive MF were decreased. Especially, polygonal HSC were abundantly observed in same hepatic areas of Rhacophorus viridis amamiensis and Rhacophorus owstoni in tree frogs. Conclusions: The activated HSC serves for metabolism of vitamin A and structural support of sinusoid, is predominantly responsible for the increased synthesis of the extracellular matrix protein during hepatic organogenesis and fibrogenesis. The present study indicates that there are differences in the pattern of HSC in the amphibians examined at this time. The usefulness in space utilization experiment was examined., 資料番号: AA0046917018},
 pages = {53--56},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)},
 title = {両生類における肝臓の肝星細胞(HSC)の比較組織化学的研究:特に系統発生学および棲息域との相関},
 year = {2004}
}