@inproceedings{oai:jaxa.repo.nii.ac.jp:00013840,
 author = {藤井, 伸治 and 清水, 美順 and 鈴木, 圭太 and 高橋, 秀幸 and Fujii, Nobuharu and Shimizu, Minobu and Suzuki, Keita and Takahashi, Hideyuki},
 book = {宇宙利用シンポジウム　第20回　平成15年度, Space Utilization Research: Proceedings of the Twentieth Space Utilization Symposium},
 month = {Mar},
 note = {Cucumber seedlings have potential to develop two pegs on the transition zone between the hypocotyl and root. Seedlings grown in a horizontal position suppress the development of the peg on the upper side of the transition zone in response to gravity. To understand how the response to gravity suppresses peg formation, cucumber mRNAs were screened by fluorescent differential display (FDD) to identify the mRNA that accumulates more in the non-peg side than in the peg side. First, to detect RT-PCR products from cucumber RNA the condition of FDD method was improved. Then, CsGRP1 cDNA that encodes glycine-rich protein was isolated using the modified condition of FDD method. Northern blotting showed that patterns of CsGRP1 mRNA accumulation corresponded to the results of FDD; namely that CsGRP1 mRNA accumulated more in the non-peg side than that in the peg side of the transition zone when peg initiation occurs. However, gravitropically bending hypocotyls did not show such differential expression of CsGRP1. These results suggest that CsGRP1 is possibly involved in gravimorphogenesis including the suppression of peg formation of cucumber seedlings., 資料番号: AA0046917035},
 pages = {106--109},
 publisher = {宇宙航空研究開発機構宇宙科学研究本部, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)},
 title = {キュウリ芽ばえの新規重力応答遺伝子cDNAの単離},
 year = {2004}
}