{"created":"2023-06-20T14:46:17.551931+00:00","id":13841,"links":{},"metadata":{"_buckets":{"deposit":"7876c658-ae4e-437a-8da6-1f4237522320"},"_deposit":{"created_by":1,"id":"13841","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13841"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013841","sets":["1543:1633:1647","1887:1891"]},"author_link":["127092","127095","127094","127093"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Gravity-regulated proteins of plants detected by 2-D electrophoresis"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"113","bibliographicPageStart":"110","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第20回　平成15年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Twentieth Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Gravity exerts a profound influence on plant growth. In gravity response of plants, gravitropism and gravimorphogenesis are known well. In the study of gravity response in plants, gravity signal transduction and transmission and expression analysis of protein-level are poorly reported. Cucurbit seedlings have unique gravity response as gravimorphogenesis. Namely, cucurbit seedlings develop a peg on the transition zone between the hypocotyls and root. Seedlings grown in a horizontal position on the ground suppress the development of the peg (negatively-controlled) on the upper side of the transition zone in response to gravity. Gravity-regulated protein expression was analyzed by two-dimensional (2-D) gel electrophoresis. As a result, the expression of 26kDa/pI5.5 and 90kDa/pI8.5 protein spots was more abundant in the lower (peg-developed) side than the upper (peg-suppressed) side of the transition zone. On the other hand, the expression of 44kDa/pI7.5 protein was more reduced in the lower side than the upper side of the transition zone. Next, to isolate putative genes cording protein spots, Arabidopsis and other plants protein databases were referred. As a result, isolated were a novel auxin-inducible gene, CsIAA4 and CsIAA3 as a transcript of 26kDa/pI5.5 protein and cell wall and glyoxylate cycle related genes, Csxylosidase and Csisocitrate lyase (CsICL), respectively, as a transcript of 90kDa/pI8.5 protein. The accumulation of CsIAA4, CsIAA3, Csxylosidase and CsICL mRNAs was more abundant in the lower side than the upper side of the transition zone. Furthermore, these genes expression of tissue specificity and gravity dependability was analyzed using 3-D clinostat. Especially, it was found that CsICL gene expression was tissue specific and only existed in the transition zone. Furthermore, the accumulation of CsIAA4, Csxylosidase and CsICL mRNAs was suppressed by clinorotation, but the accumulation of CsIAA3 mRNA increased by clinorotation. These data suggest that auxin, cell wall and glyoxylate cycle related genes were bearing the important and complicated role in gravity response of cucumber plants.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0046917036","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構宇宙科学研究本部"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部"},{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency Institute of Space and Astronautical Science"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency Institute of Space and Astronautical Science"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鎌田, 源司"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高沖, 宗夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamada, Motoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takaoki, Muneo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"電気泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"植物","subitem_subject_scheme":"Other"},{"subitem_subject":"重力効果","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質","subitem_subject_scheme":"Other"},{"subitem_subject":"植生成長","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子発現","subitem_subject_scheme":"Other"},{"subitem_subject":"重力形態形成","subitem_subject_scheme":"Other"},{"subitem_subject":"クリノローテーション","subitem_subject_scheme":"Other"},{"subitem_subject":"electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"plant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gravitational effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vegetation growth","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gravimorphogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"clinorotation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"2次元電気泳動を用いた植物の重力応答に機能する新規分子の探索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"2次元電気泳動を用いた植物の重力応答に機能する新規分子の探索"}]},"item_type_id":"5","owner":"1","path":["1647","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13841","relation_version_is_last":true,"title":["2次元電気泳動を用いた植物の重力応答に機能する新規分子の探索"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T05:43:55.305004+00:00"}