@inproceedings{oai:jaxa.repo.nii.ac.jp:00013908,
 author = {唐田, 清伸 and 菅谷, 茂 and 喜多, 和子 and 鈴木, 信夫 and Karata, Kiyonobu and Sugaya, Shigeru and Kita, Kazuko and Suzuki, Nobuo},
 book = {宇宙利用シンポジウム　第19回　平成14年度, Space Utilization Research: Proceedings of the Nineteenth Space Utilization Symposium},
 month = {Feb},
 note = {It was previously found that protease activation just after UV irradiation is induced in cultured human UVAP-1 cells. It was here investigated whether UV-activated protease activity is affected by exposure of human UVAP-1 cells to gravity-changing stress prior to UV irradiation. Levels of UV-induced protease activity were found to be increased to more extent in UVAP-1 cells exposed to drop shaft prior to UV irradiation than in cells exposed to UV irradiation alone. The cell lysate that was exposed to mock-treatment, gravity-changing stress alone, UV irradiation alone and gravity-changing stress plus UV irradiation, was partially purified by TSKgel G2000SW column chromatography. Some fractions that show high protease activity were detected in separated samples treated with both gravity-changing stress and UV irradiation. Protease activities of the fractions were effectively inhibited by cysteine protease inhibitor, E-64. To purify E-64 sensitive protease, E-64 affinity column chromatography was carried out using the cell lysate treated with both of gravity-changing stress and UV irradiation. The identification and analysis of purified proteases are in progress., 資料番号: AA0045438001},
 pages = {1--2},
 publisher = {宇宙科学研究所, The Institute of Space and Astronautical Science (ISAS)},
 title = {微小重力応答プロテアーゼの検索},
 year = {2003}
}