{"created":"2023-06-20T14:46:21.967085+00:00","id":13908,"links":{},"metadata":{"_buckets":{"deposit":"d4ec9f71-6b43-49cc-9cd4-a4aed6fd5a88"},"_deposit":{"created_by":1,"id":"13908","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"13908"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00013908","sets":["1543:1633:1648","1887:1891"]},"author_link":["127725","127722","127728","127724","127729","127726","127723","127727"],"item_5_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Search for the proteases which response under microgravity conditions"}]},"item_5_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"2","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"宇宙利用シンポジウム　第19回　平成14年度"},{"bibliographic_title":"Space Utilization Research: Proceedings of the Nineteenth Space Utilization Symposium","bibliographic_titleLang":"en"}]}]},"item_5_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"It was previously found that protease activation just after UV irradiation is induced in cultured human UVAP-1 cells. It was here investigated whether UV-activated protease activity is affected by exposure of human UVAP-1 cells to gravity-changing stress prior to UV irradiation. Levels of UV-induced protease activity were found to be increased to more extent in UVAP-1 cells exposed to drop shaft prior to UV irradiation than in cells exposed to UV irradiation alone. The cell lysate that was exposed to mock-treatment, gravity-changing stress alone, UV irradiation alone and gravity-changing stress plus UV irradiation, was partially purified by TSKgel G2000SW column chromatography. Some fractions that show high protease activity were detected in separated samples treated with both gravity-changing stress and UV irradiation. Protease activities of the fractions were effectively inhibited by cysteine protease inhibitor, E-64. To purify E-64 sensitive protease, E-64 affinity column chromatography was carried out using the cell lysate treated with both of gravity-changing stress and UV irradiation. The identification and analysis of purified proteases are in progress.","subitem_description_type":"Other"}]},"item_5_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0045438001","subitem_description_type":"Other"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙科学研究所"}]},"item_5_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"The Institute of Space and Astronautical Science (ISAS)"}]},"item_5_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"千葉大学 大学院医学研究院"},{"subitem_text_value":"千葉大学 大学院医学研究院"},{"subitem_text_value":"千葉大学 大学院医学研究院"},{"subitem_text_value":"千葉大学 大学院医学研究院"}]},"item_5_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Chiba University Graduate School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Chiba University Graduate School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Chiba University Graduate School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Chiba University Graduate School of Medicine"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"唐田, 清伸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"菅谷, 茂"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"喜多, 和子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 信夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Karata, Kiyonobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugaya, Shigeru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kita, Kazuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテアーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"紫外線照射","subitem_subject_scheme":"Other"},{"subitem_subject":"ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"落下塔","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒト細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"クロマトグラフィ","subitem_subject_scheme":"Other"},{"subitem_subject":"親和力","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ultraviolet irradiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"drop tower","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chromatography","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"affinity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference paper","resourceuri":"http://purl.org/coar/resource_type/c_5794"}]},"item_title":"微小重力応答プロテアーゼの検索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"微小重力応答プロテアーゼの検索"}]},"item_type_id":"5","owner":"1","path":["1648","1891"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"13908","relation_version_is_last":true,"title":["微小重力応答プロテアーゼの検索"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T05:42:52.551060+00:00"}