@inproceedings{oai:jaxa.repo.nii.ac.jp:00013937,
 author = {上田, 純一 and 一橋, 礼子 and 宮本, 健助 and 星野, 友紀 and 谷本, 英一 and 山下, 雅道 and Ueda, Junichi and Hitotsubashi, Reiko and Miyamoto, Kensuke and Hoshino, Tomoki and Tanimoto, Eiichi and Yamashita, Masamichi},
 book = {宇宙利用シンポジウム　第19回　平成14年度, Space Utilization Research: Proceedings of the Nineteenth Space Utilization Symposium},
 month = {Feb},
 note = {Auxin polar transport of etiolated pea (Pisum sativum L. cv. Alaska) epicotyls grown true microgravity conditions in space has shown to be reduced as compared to that in 1 g conditions. Simulated microgravity conditions on a three-dimensional clinostat and the application of inhibitor of auxin polar transport, 2,3,5-triiodobenzoic acid (TIBA), have also been effective to reduce the auxin polar transport. Strenuous effort to learn how gravity contributes to the auxin polar transport in etiolated pea epicotyls in molecular levels resulted in successful identification of putative PsPIN1 and PsAUX1 genes encoding efflux and influx carrier proteins of auxin located in plasma membrane, respectively. PsPIN1 gene was isolated using RT-PCR techniques and cloned by TA cloning method. Significantly high homology was found on nucleotide and deduced amino acid sequences among PsPIN1 and AtPIN1 to AtPIN7 genes. Expression of PsPIN1 and PsAUX1 genes in etiolated pea epicotyls observed by RT-PCR analysis was not affected by simulated microgravity conditions but promoted significantly in root tip of the seedlings. The application of PCIB (anti-auxin, p-chlorophenoxyisobutylic acid) suppressed expression of PsPIN1 and PsAUX1 genes but TIBA had almost no effect. Based on the data described above, a possible role of PsPIN1 and PsAUX1 for auxin polar transport in etiolated pea seedlings will be discussed., 資料番号: AA0045438030},
 pages = {98--101},
 publisher = {宇宙科学研究所, The Institute of Space and Astronautical Science (ISAS)},
 title = {高等植物におけるオーキシン極性移動関連遺伝子の発現に対する擬似微小重力の影響},
 year = {2003}
}