{"created":"2023-06-20T14:35:57.847427+00:00","id":2258,"links":{},"metadata":{"_buckets":{"deposit":"0921a2ee-2406-497c-b374-2c5e7f9df34d"},"_deposit":{"created_by":1,"id":"2258","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2258"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00002258","sets":["1887:1893","9:10:256:276"]},"author_link":["4727","4724","4728","4729","4725","4726"],"item_3_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"RNA interference and protein phosphorylation in space environment using the nematode Caenorhabditis elegans"}]},"item_3_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographicVolumeNumber":"JAXA-RR-05-033","bibliographic_titles":[{"bibliographic_title":"宇宙航空研究開発機構研究開発報告: 平成17年度ISS科学プロジェクト室細胞生物研究プロジェクト研究開発報告"},{"bibliographic_title":"JAXA Research and Development Report: FY 2005 Cell Biology Research Project at ISS Science Project Group Research and Development Report","bibliographic_titleLang":"en"}]}]},"item_3_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、モデル生物の1つである線虫C.elegansを用いて、微小重力や宇宙放射線をはじめとする複合的な宇宙環境ストレスが生物に及ぼす影響について調べることを目的としている。(1)我々はこれまでに、国際宇宙線虫実験ICE-first(2004年4月フライト)に参画し、宇宙で成長した線虫においても地上の対照区と同様に、減数分裂チェックポイント制御下のアポトーシスならびに卵母細胞成熟に伴う発生制御下のアポトーシスがともに正常に行われること、(2)筋肉関連の幾つかの遺伝子群ならびにタンパク質が発現レベルで顕著に低下することを見出してきた。(3)また、過重力による影響は受精直後の卵核の減数第1・第2分裂に最も影響を及ぼすことを証明してきた。(4)さらに、様々な環境ストレス(放射線など)の生物影響について、各種突然変異体やRNA干渉法(RNA interference: RNAi)による特異的な遺伝子発現の抑制体とDNAマイクロアレイを用いることにより、ゲノムワイドな分子モニターができることを明らかにしてきた。これらの結果から、複合的な宇宙環境ストレスが個体レベルに及ぼす分子影響を解析する上で、モデル生物の線虫が大変有効であることが示唆された。今後は、第1に宇宙環境におけるRNA干渉機構(RNAi)の効果を検証し、RNAiによりDNA損傷のチェックポイント制御を抑制した場合における細胞内での諸変化について調べる。さらに、アクチンや細胞骨格の再構築を介し、重力感受にも関わる可能性が想定されるRho-guanine nucleotide exchange factor(RhoGEF)の一変異体であるunc-73と野生株N2との遺伝子およびタンパク質発現の変化を比較し、シグナル伝達を含めたタンパク質のリン酸化に対する宇宙環境の影響について明らかにしたい。また、宇宙環境下で長期間、連続的な世代交代を通して生じる適応と変異・進化の方向性について明らかにすることを次なる研究課題として位置づけている。","subitem_description_type":"Abstract"}]},"item_3_description_17":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"It is important for human life in space to study the effects of environmental factors during spaceflight on a number of physiological phenomena. In the ICE-first experiment (International Caenorhabditis elegans Experiment-1, Mission DELTA, April 2004), we found that (1) both pachytene-checkpoint apoptosis and physiological apoptosis in germ cells occurred normally under spaceflight conditions and (2) certain genes related to signaling pathway of G-protein coupled receptor protein and to locomotory behavior tended to decrease in the spaceflown C. elegans. (3) In the hypergravity condition, we determined that the most susceptible aspect in the life cycle of this organism is the oocyte meiotic division for exclusion of polar bodies and the anterior-posterior polarization shortly after fertilization. (4) We also found several genes specifically responding to environmental stresses such as radiation by transcriptome analyses of entire genome using DNA microarray in several mutants and RNA interference (RNAi) animals. Since these phenomena and the identified genes are highly conserved in mammalian systems, the model experiments using this organism are useful to study the effects of space environment on human life. We are now attempting to study the RNAi effect, signal transduction, adaptation and evolution responding to space environment in future space experiments using the nematode C. elegans.","subitem_description_type":"Other"}]},"item_3_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: AA0049199001","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"レポート番号","attribute_value_mlt":[{"subitem_description":"レポート番号: JAXA-RR-05-033","subitem_description_type":"Other"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"宇宙航空研究開発機構"}]},"item_3_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"Japan Aerospace Exploration Agency (JAXA)"}]},"item_3_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-1113","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1192675X","subitem_source_identifier_type":"NCID"}]},"item_3_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東北大学 大学院生命科学研究科"},{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部"},{"subitem_text_value":"宇宙航空研究開発機構 宇宙科学研究本部"}]},"item_3_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Tohoku University Graduate School of Life Sciences"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency Institute of Space and Astronautical Science"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency Institute of Space and Astronautical Science"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"東谷, 篤志"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"東端, 晃"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石岡, 憲昭"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higashitani, Atsushi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higashibata, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishioka, Noriaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-15"}],"displaytype":"detail","filename":"49199001.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"49199001.pdf","url":"https://jaxa.repo.nii.ac.jp/record/2258/files/49199001.pdf"},"version_id":"83971937-2dea-42ae-8f33-54f673a8279f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"線虫","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスクリプトーム","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオーム","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA干渉","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙生物学","subitem_subject_scheme":"Other"},{"subitem_subject":"アポトーシス","subitem_subject_scheme":"Other"},{"subitem_subject":"宇宙飛行","subitem_subject_scheme":"Other"},{"subitem_subject":"微小重力","subitem_subject_scheme":"Other"},{"subitem_subject":"高重力環境","subitem_subject_scheme":"Other"},{"subitem_subject":"リン酸化","subitem_subject_scheme":"Other"},{"subitem_subject":"シグナル伝達","subitem_subject_scheme":"Other"},{"subitem_subject":"caenorhabditis elegans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transcriptome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proteome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA interference","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"exobiology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"space flight","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high gravity environment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphorylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"signal transduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"technical report","resourceuri":"http://purl.org/coar/resource_type/c_18gh"}]},"item_title":"線虫C.elegansを用いた宇宙環境におけるRNAiとタンパク質リン酸化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"線虫C.elegansを用いた宇宙環境におけるRNAiとタンパク質リン酸化"}]},"item_type_id":"3","owner":"1","path":["276","1893"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-26"},"publish_date":"2015-03-26","publish_status":"0","recid":"2258","relation_version_is_last":true,"title":["線虫C.elegansを用いた宇宙環境におけるRNAiとタンパク質リン酸化"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T09:25:46.771409+00:00"}