{"created":"2023-06-20T14:54:19.905239+00:00","id":22653,"links":{},"metadata":{"_buckets":{"deposit":"6cbad1d4-e8bf-4e7a-ac21-7079cdcd98cb"},"_deposit":{"created_by":1,"id":"22653","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"22653"},"status":"published"},"_oai":{"id":"oai:jaxa.repo.nii.ac.jp:00022653","sets":["1887:1888"]},"author_link":["212271","212267","212270","212268","212266","212264","212265","212269","212272","212273"],"item_7_alternative_title_2":{"attribute_name":"その他のタイトル（英）","attribute_value_mlt":[{"subitem_alternative_title":"Evaluation of Cellular Effects Caused by Lunar Regolith Simulant Including Fine Particles"}]},"item_7_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"148","bibliographicPageStart":"139","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"産業医科大学雑誌"},{"bibliographic_title":"Journal of UOEH","bibliographic_titleLang":"en"}]}]},"item_7_description_16":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"アメリカ航空宇宙局による有人月面探査構想が発表され，我が国も参加を表明している．月面には，月レゴリスと呼ばれる微粒子を含む表土が堆積しており，月面での活動によって月レゴリスが舞い上がり，宇宙船内に持ち込まれて吸入する可能性がある． 月面探査に先立ち，月レゴリスの有害性評価が必要であることから，本研究では月レゴリスの化学組成と粒径に着目し，細胞に対する影響を検討した．アポロが持ち帰った月レゴリスと同様の組成を持つ月レゴリスシミュラントを用い，液相沈降法により粒径が10 μm以下，25 μm以下および10～25 μm となるように分画した．さらに，平均粒子径5.10 μmの微粒子からなる月レゴリスシミュラントを用い，0.1および1.0mg/ml の濃度でヒト肺胞上皮腺癌由来のA549細胞およびマクロファージ様に分化させたヒト単球由来のTHP-1細胞に投与し，24時間後に，細胞傷害，酸化ストレス誘導および炎症誘導性を評価した．濃度1.0 mg/ml のときには，細胞膜の損傷とミトコンドリア活性の低下と，インターロイキン- 8（ IL-8）の分泌および遺伝子発現の上昇が認められたが，0.1 mg/mlではこれらの影響はないか，ごく小さく，陽性対照とした結晶性SiO2に比べても影響は小さかった．THP-1細胞において，濃度1.0 mg/mlでは培養上清中にIL-1βおよび腫瘍壊死因子-α（ TNF-α）の分泌がみられたが，24時間の時点でIL-1βおよびTNF-α遺伝子の上昇はみられなかった．また，細胞内酸化ストレスの上昇も小さかった．細胞膜損傷，酸化ストレス誘発あるいは炎症誘発性といった細胞影響は＜ 10 μmの画分で強い傾向があったが，顕著な違いはなかった．以上の結果から，in vitroの試験において化学組成の面からの細胞影響は小さく，粒子径の影響もそれほど大きくないことが示された．","subitem_description_type":"Abstract"}]},"item_7_description_18":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"形態: 図版あり","subitem_description_type":"Other"}]},"item_7_description_19":{"attribute_name":"内容記述（英）","attribute_value_mlt":[{"subitem_description":"Physical characteristics: Original contains illustrations","subitem_description_type":"Other"}]},"item_7_description_32":{"attribute_name":"資料番号","attribute_value_mlt":[{"subitem_description":"資料番号: PA1610023000","subitem_description_type":"Other"}]},"item_7_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"産業医科大学"}]},"item_7_publisher_9":{"attribute_name":"出版者（英）","attribute_value_mlt":[{"subitem_publisher":"University of Occupational and Environmental Health"}]},"item_7_relation_25":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.7888/juoeh.37.139"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.7888/juoeh.37.139","subitem_relation_type_select":"DOI"}}]},"item_7_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0387-821X","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_24":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0009832X","subitem_source_identifier_type":"NCID"}]},"item_7_text_6":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"産業医科大学産業生態科学研究所: 産業技術総合研究所(AIST)"},{"subitem_text_value":"宇宙航空研究開発機構(JAXA)"},{"subitem_text_value":"宇宙航空研究開発機構(JAXA)"},{"subitem_text_value":"宇宙航空研究開発機構(JAXA)"},{"subitem_text_value":"産業医科大学産業生態科学研究所"}]},"item_7_text_7":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health: National Institute of Advanced Industrial Science and Technology (AIST)"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Japan Aerospace Exploration Agency (JAXA)"},{"subitem_text_language":"en","subitem_text_value":"Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"堀江, 祐範"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三木, 猛生"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"本間, 善之"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"青木, 滋"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森本, 泰夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Horie, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miki, Takeo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honma, Yoshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aoki, Shigeru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morimoto, Yasuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"月レゴリスシミュラント","subitem_subject_scheme":"Other"},{"subitem_subject":"微粒子","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞傷害","subitem_subject_scheme":"Other"},{"subitem_subject":"酸化ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"炎症","subitem_subject_scheme":"Other"},{"subitem_subject":"lunar regolith simulant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fine particle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytotoxicity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oxidative stress","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"inflammation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"微粒子を含む月レゴリスシミュラントの細胞影響の評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"微粒子を含む月レゴリスシミュラントの細胞影響の評価"}]},"item_type_id":"7","owner":"1","path":["1888"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-06-13"},"publish_date":"2016-06-13","publish_status":"0","recid":"22653","relation_version_is_last":true,"title":["微粒子を含む月レゴリスシミュラントの細胞影響の評価"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-21T03:17:33.108658+00:00"}